Pollen Cryobanking for Tropical Plant Species
Pollen Cryobanking for Tropical Plant Species
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热带植物花粉冷冻银行
DOI：
10.1007/978-1-4614-3776-5_4
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发表时间：
2013
影响因子：
--
通讯作者：
S. Ganeshan
中科院分区：
文献类型：
--
作者：
P. Rajasekharan;B. Ravish;T. Kumar;S. Ganeshan
储存花粉以备将来使用是野生作物近缘物种保护遗传资源的重要工具。大多数植物的成熟雄配子体具有耐干燥性，这使它们非常适合于储存。花粉冷冻保存受威胁植物的一个主要影响将是保持遗传多样性的股票花粉收集从野生的未来保护措施，一个战略，也可以实现保护核遗传多样性（NGD），以保护通过花粉表达的遗传变异。这是在自然情况下唯一可以利用的变异性，特别是当它们的繁殖行为信息鲜为人知时。花粉长期超低温保存已在许多种植物中得到成功应用。案例研究花粉从五个RET（稀有，濒危，受威胁）植物，需要立即保护注意，即，研究了南蛇藤、乌藨子、木蝴蝶、十鳞草花粉和全缘木花粉的超低温保存适应性。这些物种的花粉/花粉在初始活力评估后通过直接浸入液氮中冷冻保存。冷冻保存1周后，重新评估花粉/花粉块的活力。三个物种在冷冻保存后的活力没有差异。剩余的两个物种下降后冷冻保存的活力减少发芽的方式相比，他们的新鲜的同行。在这两个物种与花粉有一个延迟后存储萌发启动1和2小时，相比，新鲜花粉的初始萌发。结果表明，对于本研究中研究的RET植物，可以鼓励花粉冷冻保存以保存核遗传变异性，其可以以增加的效率用于提高种子结实率以用于物种恢复、扩增等，生态修复/生态恢复计划。
Storing pollen for future use is an important tool in species that are wild crop relatives, for conservation of genetic resources. The mature male gametophyte of most plant species is desiccation tolerant which render them ideally suitable for storage. A major impact of pollen cryopreservation of threatened plants will be to maintain genetically diverse stocks of pollen collected from wild for future conservation measures, a strategy which can also accomplish conservation of nuclear genetic diversity (NGD) to conserve genetic variation expressed through pollen. This is the only variability that could be tapped under natural situations, especially when information on their breeding behavior is less known. Long term pollen cryopreservation has been attempted successfully in many species for conservation of NGD. Case studies of pollen from five RET (rare, endangered, threatened) plants which require immediate conservation attention viz., Celastrus paniculatus, Cayratia pedata, Oroxylum indicum and pollenia from Decalepis hamiltonii and Holostemma adakodien were investigated for their suitability to cryogenic preservation. Pollen/pollinia of these species after initial viability assessment were cryopreserved by direct immersion in liquid nitrogen. After 1 week of cryostorage, pollen/pollinia were re-assessed for viability. Three species showed no difference in post cryostorage viability. The remaining two species declined in post-cryostorage viability by way of reduced germination when compared to their fresh counterparts. Among the two species with pollinia there was a delayed post-storage germination initiation by 1 and 2 h, compared to initial germination of fresh pollinia. The results indicate that for RET plants investigated in this study, pollen cryopreservation can be encouraged to conserve nuclear genetic variability which could be used with increased efficiency to improve seed set for species recovery, amplification, etc., for eco-rehabilitation/eco-restoration programs.
植物育种评论
DOI：
10.1002/9781119828235.ch5
发表时间：
2021
期刊：
--
影响因子：
--
作者：
Blair M
通讯作者：
Blair M