A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa.
A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa.
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DOI：
10.1038/ncomms1153
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发表时间：
2011-01-11
影响因子：
16.6
通讯作者：
Clapham DE
中科院分区：
文献类型：
--
作者：
Chung JJ;Navarro B;Krapivinsky G;Krapivinsky L;Clapham DE
钙信号是成功受精的关键。在精子中，获能、运动过度激活和顶体反应都是由细胞内Ca 2+的增加介导的。精子蛋白的阳离子通道（CATSPERS 1 -4）形成精子的超活化运动性和男性生育力所需的碱化激活的Ca 2+选择性通道。每个CatSper 1 -4基因编码围绕Ca 2+选择性孔的四聚体的亚基，类似于其他六跨膜离子通道α亚基。除了成孔蛋白外，精子Ca 2+通道还含有辅助亚基CATSPERβ和CATSPERγ。在这里，我们确定Tmem 146基因产物作为一个新的亚基，CATSPERδ，所需的CATSPER通道功能。我们发现，缺乏精子尾部特异性CATSPERδ的小鼠是不育的，它们的精子缺乏钙电流和超活化运动。我们表明，CATSPERδ是CATSPER通道复合物的基本要素，并建议CATSPERδ是正确的CATSPER通道组装和/或运输所必需的。
Calcium signaling is critical for successful fertilization. In spermatozoa, capacitation, hyperactivation of motility, and the acrosome reaction are all mediated by increases in intracellular Ca2+. Cation channels of sperm proteins (CATSPERS1-4) form an alkalinization-activated Ca2+-selective channel required for the hyperactivated motility of spermatozoa and male fertility. Each of the CatSper1-4 genes encodes a subunit of a tetramer surrounding a Ca2+-selective pore, in analogy with other six-transmembrane ion channel α subunits. In addition to the pore-forming proteins, the sperm Ca2+ channel contains auxiliary subunits, CATSPERβ and CATSPERγ. Here, we identify the Tmem146 gene product as a novel subunit, CATSPERδ, required for CATSPER channel function. We find that mice lacking the sperm tail-specific CATSPERδ are infertile and their spermatozoa lack both Ca2+ current and hyperactivated motility. We show that CATSPERδ is an essential element of the CATSPER channel complex and propose that CATSPERδ is required for proper CATSPER channel assembly and/or transport.
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影响因子：
1.9
作者：
Ho, Katharine;Wolff, Collin A.;Suarez, Susan S.
通讯作者：
Suarez, Susan S.
DOI：
10.1073/pnas.0400050101
发表时间：
2004-03-02
影响因子：
11.1
作者：
Esposito, G;Jaiswal, BS;Gossen, JA
通讯作者：
Gossen, JA
DOI：
10.1073/pnas.2536658100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Carlson, AE;Westenbroek, RE;Babcock, DF
通讯作者：
Babcock, DF
DOI：
10.1073/pnas.0610286104
发表时间：
2007-01-23
影响因子：
11.1
作者：
Qi, Huayu;Moran, Magdalene M.;Clapham, David E.
通讯作者：
Clapham, David E.
影响因子：
3.5
作者：
Santi CM;Martínez-López P;de la Vega-Beltrán JL;Butler A;Alisio A;Darszon A;Salkoff L
通讯作者：
Salkoff L