Microfluidic confinement of single cells of bacteria in small volumes initiates high-density behavior of quorum sensing and growth and reveals its variability.
Microfluidic confinement of single cells of bacteria in small volumes initiates high-density behavior of quorum sensing and growth and reveals its variability.
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DOI：
10.1002/anie.200901550
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发表时间：
2009
影响因子：
16.6
通讯作者：
Ismagilov, Rustem F.
中科院分区：
文献类型：
--
作者：
Boedicker, James Q.;Vincent, Meghan E.;Ismagilov, Rustem F.
在此，我们表明，在小体积的限制，铜绿假单胞菌的细菌，包含少至一到三个细胞，能够启动群体感应（QS），并实现QS依赖性的增长。此外，我们表明，在低数量的细胞，启动QS是高度可变的克隆人口内。QS途径参与微生物的关键功能，例如发病机制、生物膜的形成、孢子形成、营养物质的获取、接合、运动和次级代谢产物（例如抗生素）的产生。[1]通过限制对QS途径进行实验性控制，可以对QS在小细胞群中的作用进行基础研究，并可以为培养不可培养的细菌或诱导抗生素生产提供工具。QS根据定义是一种高密度行为，由每单位体积的细胞数量调节。QS由释放的信号分子（如自诱导物（AI））的积累启动，并且已知当细胞密度上升到阈值水平以上时启动。有两种方法可以激活培养物中的高密度行为：1）用细菌接种宏观体积，并让它们分裂，直到它们达到高密度，或2）取一些细胞并将它们限制在非常小的体积中，以使AI积聚（图1A）。通过策略（1）激活QS占主导地位，并导致普遍认为QS是一个协调大群细胞集体行为的过程，[2，3]而小群细菌在限制时启动QS的可能性经常被忽视。然而，策略（2）是重要的考虑，因为QS途径是相关的功能，生存和少量细胞的生长，例如，在生物膜形成的早期，在感染的早期阶段，或在土壤中。
Herein we show that upon confinement in small volumes, groups of Pseudomonas aeruginosa bacteria, containing as few as one to three cells, are able to initiate quorum sensing (QS) and achieve QS-dependent growth. In addition, we show that, at low numbers of cells, initiation of QS is highly variable within a clonal population. QS pathways are involved in critical functions of microorganisms, such as pathogenesis, development of biofilms, sporulation, acquisition of nutrients, conjugation, motility, and production of secondary metabolites such as antibiotics.[1] Experimental control of QS pathways through confinement may enable fundamental research into the role of QS in small groups of cells and could provide a tool for growing unculturable bacteria or inducing antibiotic production.QS is by definition a high-density behavior, regulated by the number of cells per unit volume. QS is initiated by the accumulation of released signaling molecules, such as auto-inducers (AIs), and is known to be initiated when the density of cells rises above a threshold level. There are two approaches to activating high-density behavior in cultures: 1) seed a macroscopic volume with bacteria and let them divide until they reach high density, or 2) take a few cells and confine them in a very small volume to enable accumulation of AIs (Figure 1A). Activation of QS through strategy (1) has dominated and has led to the general view that QS is a process to coordinate the collective behavior of large groups of cells,[2, 3] and the possibility that small groups of bacteria could initiate QS upon confinement is often overlooked. Nevertheless, strategy (2) is important to consider, because QS pathways are relevant to function, survival, and growth of small numbers of cells—for example, early in biofilm formation, in early stages of infection, or in soils.
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影响因子：
5.8
作者：
Parent, Mary E.;Snyder, Charles E.;Velegol, Darrell
通讯作者：
Velegol, Darrell
影响因子：
6.1
作者：
Faley S;Seale K;Hughey J;Schaffer DK;VanCompernolle S;McKinney B;Baudenbacher F;Unutmaz D;Wikswo JP
通讯作者：
Wikswo JP
影响因子：
3.2
作者：
Kirisits, Mary Jo;Margolis, Jeffrey J.;Parsek, Matthew R.
通讯作者：
Parsek, Matthew R.
DOI：
10.1073/pnas.0711723105
发表时间：
2008-02-26
影响因子：
11.1
作者：
Dulla, Glenn;Lindow, Steven E.
通讯作者：
Lindow, Steven E.
影响因子：
7.4
作者：
Ahn, S;Walt, DR
通讯作者：
Walt, DR