Upregulation of SEPT7 Gene Inhibits Invasion of Human Glioma Cells
Upregulation of SEPT7 Gene Inhibits Invasion of Human Glioma Cells
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SEPT7基因的上调抑制人胶质瘤细胞的侵袭
DOI：
10.3109/07357900903179609
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发表时间：
2010-02
影响因子：
2.4
通讯作者：
Xu, Peng
中科院分区：
文献类型：
--
作者：
Xu, Song;Kang, Chunsheng;Liu, Xiaozhi;Huang, Giang;Wang, Guangxiu;Jia, Zhi-Fan;Pu, Peiyu;Zhou, Xuan;Xu, Peng
【摘要】目的：探讨SEPT7在胶质瘤细胞侵袭中的作用。方法：将SEPT7转染人胶质瘤细胞系U251和TJ899，采用transwell法、划痕法和3-D/2-D Matrigel生长法评价其侵袭能力。免疫组化、免疫荧光、Western blot检测MMP2/9、MT1-MMP、整合素αvβ3、TIMP1/2的表达。激光扫描共聚焦分析α-微管蛋白的分布。结果：转染SEPT7后，细胞侵袭受到抑制，MMP2/9、MT1-MMP、整合素αvβ3表达降低，TIMP1/2表达升高，α-微管蛋白重新分布。结论：这些结果提示SEPT7在胶质瘤细胞侵袭中起重要作用。
ABSTRACT Objectives: To explore the role of SEPT7 in glioma cell invasion. Methods: SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin αvβ3, and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of α-tubulin was examined by laser scanning confocal analysis. Result: After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin αvβ3 was decreased, while TIMP1/2 was increased, and α-tubulin was redistributed. Conclusion: These results suggest that SEPT7 plays an important role in the glioma cell invasion.
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影响因子：
--
作者：
H. Strander
通讯作者：
H. Strander
影响因子：
158.5
作者：
Stupp, R;Mason, WP;Ryan, G
通讯作者：
Ryan, G
影响因子：
7.3
作者：
Burrows, JF;Chanduloy, S;Russell, SHE
通讯作者：
Russell, SHE
影响因子：
11.4
作者：
Kojima, K;Sakai, I;Fujita, S
通讯作者：
Fujita, S
影响因子：
11.2
作者：
R. Ono;T. Taki;T. Taketani;H. Kawaguchi;M. Taniwaki;T. Okamura;K. Kawa;R. Hanada;Miyuki Kobayashi;Y. Hayashi
通讯作者：
R. Ono;T. Taki;T. Taketani;H. Kawaguchi;M. Taniwaki;T. Okamura;K. Kawa;R. Hanada;Miyuki Kobayashi;Y. Hayashi