Resveratrol dilates arterioles and protects against N-methyl-d-aspartic acid-induced excitotoxicity in the rat retina
Resveratrol dilates arterioles and protects against N-methyl-d-aspartic acid-induced excitotoxicity in the rat retina
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白藜芦醇可扩张小动脉并防止 N-甲基-d-天冬氨酸引起的大鼠视网膜兴奋性毒性
DOI：
10.1016/j.neulet.2022.136999
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发表时间：
2023
影响因子：
2.5
通讯作者：
Nakahara Tsutomu
中科院分区：
文献类型：
--
作者：
Mori Asami;Ezawa Yuna;Asano Daiki;Kanamori Toshiki;Morita Akane;Kashihara Toshihide;Sakamoto Kenji;Nakahara Tsutomu
白藜芦醇是一种天然多酚类化合物，具有抗炎、抗氧化等多种生物活性。在目前的研究中，我们研究了（1）白藜芦醇对视网膜小动脉的扩张作用，（2）白藜芦醇对兴奋性视网膜损伤的保护作用，以及（3）这些作用是否由AMP激活激酶（AMPK）依赖性通路介导。本研究使用雄性Wistar大鼠（7至10周龄）。在体内测量视网膜小动脉的直径、平均动脉压和心率。通过组织学检查评估视网膜损伤。静脉注射白藜芦醇（3 mg/kg）增加视网膜小动脉的直径，而不影响平均动脉压和心率。AMPK抑制剂，化合物C（5毫克/公斤，静脉注射），显着减弱视网膜血管扩张反应白藜芦醇。玻璃体内注射N-甲基-d-天冬氨酸（NMDA; 25、50和100 nmol/眼）后7天，位于神经节细胞层（GCL）的细胞数量减少，沿着内丛状层变薄。玻璃体内注射白藜芦醇（100 nmol/眼）减少NMDA（25和50 nmol/眼）诱导的GCL细胞丢失。白藜芦醇的神经保护作用被化合物C（10 nmol/眼）显著但不完全逆转。这些结果表明，白藜芦醇扩张视网膜小动脉，并通过AMPK依赖性途径保护大鼠免受NMDA诱导的视网膜神经变性。白藜芦醇可能具有通过改善受损的视网膜循环和保护视网膜神经元细胞来减缓与视网膜缺血相关的疾病的发作和进展的潜力。
Resveratrol, a natural polyphenolic compound, reportedly possesses numerous biological activities, including anti-inflammatory and antioxidant effects. In the current study, we examined (1) the dilator effects of resveratrol on retinal arterioles, (2) the protective effects of resveratrol against excitotoxic retinal injury, and (3) whether these effects are mediated by the AMP-activated kinase (AMPK)-dependent pathway in rats. Male Wistar rats (7 to 10 weeks old) were used in this study. The diameters of the retinal arterioles, mean arterial pressure, and heart rate were measured in vivo. The retinal injury was assessed by histological examination. Intravenous injection of resveratrol (3 mg/kg) increased the diameter of the retinal arterioles without affecting the mean arterial pressure and heart rate. The AMPK inhibitor, compound C (5 mg/kg, intravenously), significantly attenuated the retinal vasodilator response to resveratrol. Seven days after intravitreal injection ofN-methyl-d-aspartic acid (NMDA; 25, 50, and 100 nmol/eye), the number of cells located in the ganglion cell layer (GCL) was reduced, along with thinning of the inner plexiform layer. Intravitreal resveratrol injection (100 nmol/eye) reduced the NMDA (25 and 50 nmol/eye)-induced cell loss in the GCL. The neuroprotective effect of resveratrol was significantly but not completely reversed by compound C (10 nmol/eye). These results suggest that resveratrol dilates retinal arterioles and protects against NMDA-induced retinal neurodegeneration via an AMPK-dependent pathway in rats. Resveratrol may have the potential to slow the onset and progression of diseases associated with retinal ischemia by improving impaired retinal circulation and protecting retinal neuronal cells.
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白藜芦醇对牛视网膜动脉的血管舒张作用。
影响因子：
4.4
作者：
L. Vanden Daele;Charlotte Boydens;B. Pauwels;J. van de Voorde
通讯作者：
J. van de Voorde
影响因子：
6.2
作者：
Akiko Takeda;Youichi Shinozaki;K. Kashiwagi;N. Ohno;Kei Eto;H. Wake;J. Nabekura;S. Koizumi
通讯作者：
Akiko Takeda;Youichi Shinozaki;K. Kashiwagi;N. Ohno;Kei Eto;H. Wake;J. Nabekura;S. Koizumi
DOI：
10.1016/s0169-328x(01)00116-4
发表时间：
2001-07-13
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Laabich, A;Li, GY;Cooper, NGF
通讯作者：
Cooper, NGF
影响因子：
3.4
作者：
Ji, Kaibao;Li, Zongyuan;Xing, Yiqiao
通讯作者：
Xing, Yiqiao
影响因子：
7.7
作者：
Davis, BJ;Xie, ZL;Zou, MH
通讯作者：
Zou, MH