dRVVT Is more Sensitive than KCT or TTI for Detecting Lupus Anticoagulant Activity of Anti-β2-glycoprotein I Autoantibodies
dRVVT Is more Sensitive than KCT or TTI for Detecting Lupus Anticoagulant Activity of Anti-β2-glycoprotein I Autoantibodies
复制标题
dRVVT 检测抗 β2-糖蛋白 I 自身抗体的狼疮抗凝活性比 KCT 或 TTI 更灵敏
作者：
V. Pengo;A. Biasiolo;P. Rampazzo;T. Brocco
抗β2-糖蛋白I（β2-GPI）抗体的作用与经典的狼疮抗凝剂（LA）相似，因为它们抑制磷脂依赖性凝血反应，并且在存在过量外源性磷脂（PL）的情况下其活性消失。我们最近已经证明，在稀释的Russell Viper Venom Time（dRVVT）测试系统中需要一定量的PL来表达抗β2-GPI抗体的LA活性。我们现在已经将这一观察结果扩展到另外两个测试，即，未添加PL的高岭土凝血时间（KCT）和PL极度稀释的组织凝血活酶抑制试验（TTI）。事实上，亲和纯化的抗体制剂从5例抗磷脂综合征没有表达或只有弱表达的抗凝活性在这两个测试中，与纯化的抗体和对照缓冲液的凝血时间的平均比值分别为1.11和1.0 KCT和TTI。相反，在PL稀释度为1：8和1：64时，dRVVT的平均比值分别为1.31和1.49。因此，由于β2-GPI自身抗体导致的LA活性的存在特征为dRVVT阳性和KCT和TTI阴性或仅弱阳性。
Summary Anti-β2-glycoprotein I (β2-GPI) antibodies behave as classical Lupus Anticoagulants (LA), as they inhibit phospholipid-dependent coagulation reactions and their activity disappears in the presence of excess exogenous phospholipids (PLs). We have recently shown that a certain amount of PLs in the dilute Russell Viper Venom Time (dRVVT) test system is required to express LA activity of anti β2-GPI antibodies. We have now extended this observation to two other tests, i.e., Kaolin Clotting Time (KCT) in which PLs are not added, and Tissue Thromboplastin Inhibition test (TTI) in which PLs are extremely diluted. In fact, affinity-purified antibody preparations from 5 patients with antiphospholipid syndrome did not express or only weakly expressed anticoagulant activity in both tests; the mean ratios of coagulation times obtained with purified antibodies and that of control buffer were 1.11 and 1.0 for KCT and TTI, respectively. On the contrary, the mean ratios in dRVVT were 1.31 and 1.49 at a PLs dilution of 1:8 and 1:64, respectively. Therefore, the presence of LA activity due to autoantibodies to β2-GPI is characterized by a positive dRVVT and negative or only weakly positive KCT and TTI.
表面依赖性止血。
影响因子：
3.6
作者：
Mann,KG;Krishnaswamy,S;Lawson,JH
通讯作者：
Lawson,JH
影响因子：
20.3
作者：
P. Thiagarajan;V. Pengo;S. Shapiro
通讯作者：
P. Thiagarajan;V. Pengo;S. Shapiro