CD84 is up‐regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT‐2
CD84 is up‐regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT‐2
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CD84 在人类记忆 B 细胞的主要群体中上调，并招募含有 SAP 和 EAT-2 蛋白的 SH2 结构域
DOI：
10.1002/1521-4141(200206)32:6
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发表时间：
2002
影响因子：
5.4
通讯作者：
P. Hodgkin
中科院分区：
文献类型：
--
作者：
S. Tangye;Barbara C. M. van de Weerdt;D. Avery;P. Hodgkin
CD84是细胞表面受体免疫球蛋白超家族CD2亚群的一员。这个家族的几个成员参与T细胞和NK细胞的激活。虽然CD84最初是从人B细胞系cDNA文库中克隆出来的，但对其在人原代白细胞中的生物学作用知之甚少。我们研究了CD84在人B细胞中的表达及其生化特性。CD84在外周血、脾和脐带血B细胞上表达。脾B细胞可分离到CD84lo和CD84hi两个群体。与CD84lo B细胞相比，CD84hi B细胞代表了记忆B细胞的一个子集，这表明细胞大小增加，记忆B细胞特异性标记CD27的共表达，Ig可变区基因的体细胞突变以及增殖增加。在与特异性单克隆抗体连接后，CD84在酪氨酸残基上迅速磷酸化，并募集细胞质接头蛋白SAP和EAT‐2。CD84能够经历酪氨酸磷酸化并招募这些含有SH2结构域的蛋白，这表明它可能在B细胞，特别是记忆细胞的激活中起作用，其信号转导途径可能利用SAP和/或EAT‐2。因此，研究CD84和CD27的表达和功能可能有助于更好地了解正常和免疫功能低下宿主中记忆B细胞的发育和生物学。
CD84 is a member of the CD2 subset of the immunoglobulin superfamily of cell surface receptors. Several members of this family are involved in the activation of T cells and NK cells. Although CD84 was originally cloned from a human B cell line cDNA library, very little is known regarding its biology on primary human leukocytes. We investigated the expression and biochemistry of CD84 on human B cells. CD84 was expressed on B cells in peripheral blood, spleen and cord blood. Two populations of splenic B cells could be resolved, CD84lo and CD84hi. CD84hi B cells represented a subset of memory B cells as demonstrated by increased cell size, co‐expression of the memory B cell‐specific marker CD27, somatically mutated Ig variable region genes, and increased proliferation compared to CD84lo B cells. CD84 became rapidly phosphorylated on tyrosine residues following ligation with a specific monoclonal antibody and recruited the cytoplasmic adaptor proteins SAP and EAT‐2. The ability of CD84 to undergo tyrosine phosphorylation and to recruit these SH2 domain‐containing proteins suggests it may function in the activation of B cells, particularly memory cells, and its signal transduction pathway may utilize SAP and/or EAT‐2. Thus, investigation of expression and function of CD84 and CD27 is likely to contribute to a greater understanding of the development and biology of memory B cells in normal and immunocompromised hosts.
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影响因子：
4.4
作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
通讯作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
影响因子：
4.2
作者：
Kinsella, TM;Nolan, GP
通讯作者：
Nolan, GP
DOI：
10.1073/pnas.95.23.13765
发表时间：
1998-11-10
影响因子：
11.1
作者：
Nichols, KE;Harkin, DP;Haber, DA
通讯作者：
Haber, DA
X连锁淋巴增殖综合征基因产物SAP与CD2受体家族成员的细胞质结构域的独特相互作用。
DOI：
10.1006/clim.2001.5035
发表时间：
2001
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
--
作者：
Lewis,J;Eiben,LJ;Nelson,DL;Cohen,JI;Nichols,KE;Ochs,HD;Notarangelo,LD;Duckett,CS
通讯作者：
Duckett,CS
影响因子：
7.1
作者：
Feng, Li;Qiao, Yan;Zhou, Guanghong
通讯作者：
Zhou, Guanghong