The role of Eya1 and Eya2 in the taste system of mice from embryonic stage to adulthood.
The role of Eya1 and Eya2 in the taste system of mice from embryonic stage to adulthood.
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DOI：
10.3389/fcell.2023.1126968
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发表时间：
2023
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
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作者：
Eya家族成员是一类具有磷酸酶活性的转录因子，在发育过程中广泛表达于颅感觉器官。然而，目前还不清楚这些基因是否在发育过程中在味觉系统中表达，以及它们是否在指定味觉细胞命运中发挥任何作用。在这项研究中，我们报告说，Eya 1不表达在胚胎舌发育，但Eya 1表达的祖细胞在体节或咽内胚层引起舌肌肉组织或味觉器官，分别。在Eya 1缺陷的舌头中，这些祖细胞不能正常增殖，导致出生时舌头变小，味觉乳头生长受损，乳头上皮中Six1的表达中断。另一方面，Eya 2特异性表达于发育过程中位于舌后部的内胚层来源的轮廓状和叶状乳头。在成年人的舌头，Eya 1主要是在轮廓和叶状乳头的味蕾IP3R3阳性的味觉细胞表达，而Eya 2是持续表达在这些乳头在一些上皮祖细胞在较高的水平和在一些味觉细胞在较低的水平。我们发现在第三周条件性敲除Eya 1或Eya 2敲除减少了Pou2f3+、Six1+和IP3R3+味觉细胞。我们的数据首次定义了Eya 1和Eya 2在小鼠味觉系统发育和维持过程中的表达模式，并表明Eya 1和Eya 2可能共同作用，促进味觉细胞亚型的谱系定型。
Members of the Eya family, which are a class of transcription factors with phosphatase activity, are widely expressed in cranial sensory organs during development. However, it is unclear whether these genes are expressed in the taste system during development and whether they play any role in specifying taste cell fate. In this study, we report that Eya1 is not expressed during embryonic tongue development but that Eya1-expressing progenitors in somites or pharyngeal endoderm give rise to tongue musculature or taste organs, respectively. In the Eya1-deficient tongues, these progenitors do not proliferate properly, resulting in a smaller tongue at birth, impaired growth of taste papillae, and disrupted expression of Six1 in the papillary epithelium. On the other hand, Eya2 is specifically expressed in endoderm-derived circumvallate and foliate papillae located on the posterior tongue during development. In adult tongues, Eya1 is predominantly expressed in IP3R3-positive taste cells in the taste buds of the circumvallate and foliate papillae, while Eya2 is persistently expressed in these papillae at higher levels in some epithelial progenitors and at lower levels in some taste cells. We found that conditional knockout of Eya1 in the third week or Eya2 knockout reduced Pou2f3+, Six1+ and IP3R3+ taste cells. Our data define for the first time the expression patterns of Eya1 and Eya2 during the development and maintenance of the mouse taste system and suggest that Eya1 and Eya2 may act together to promote lineage commitment of taste cell subtypes.
RNA-seq分析有关鸡肉味感器官的分析：研究器官发生的理想系统。
影响因子：
4.6
作者：
Cui X;Marshall B;Shi N;Chen SY;Rekaya R;Liu HX
通讯作者：
Liu HX