RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis.
RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis.
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RNA-seq分析有关鸡肉味感器官的分析：研究器官发生的理想系统。
DOI：
10.1038/s41598-017-09299-7
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发表时间：
2017-08-22
影响因子：
4.6
通讯作者：
Liu HX
中科院分区：
文献类型：
--
作者：
Cui X;Marshall B;Shi N;Chen SY;Rekaya R;Liu HX
RNA-Seq是细胞和组织转录组学分析的有力工具。我们最近利用分子标记对鸡的上颚、口腔基部和后舌的口腔上皮片进行染色，发现了比以前更多的味蕾。本研究采用RNA-Seq技术了解鸡味觉组织的转录组结构。有趣的是，与上颚和后舌相比，在口腔基部的上皮和间质之间发现了味觉相关基因和更多的差异表达基因（DEGs）。进一步使用口腔底部特定组织的rna测序表明，味觉上皮（GE）和非味觉上皮（NGE）之间以及GE和底层间质（GM）之间的deg在多种GO术语和KEGG途径中富集，包括许多生物过程。众所周知的味觉基因在转基因中高度表达。此外，在器官发生过程中重要的信号通路基因（Wnt, TGFβ/ BMP， FGF, Notch， SHH, Erbb）在转基因和转基因之间的表达也存在差异。结合鸡味蕾的其他特征，如独特的模式排列和短的转换周期，我们的数据表明，鸡味觉组织为器官发生和再生医学等多学科研究提供了理想的系统。
RNA-Seq is a powerful tool in transcriptomic profiling of cells and tissues. We recently identified many more taste buds than previously appreciated in chickens using molecular markers to stain oral epithelial sheets of the palate, base of oral cavity, and posterior tongue. In this study, RNA-Seq was performed to understand the transcriptomic architecture of chicken gustatory tissues. Interestingly, taste sensation related genes and many more differentially expressed genes (DEGs) were found between the epithelium and mesenchyme in the base of oral cavity as compared to the palate and posterior tongue. Further RNA-Seq using specifically defined tissues of the base of oral cavity demonstrated that DEGs between gustatory (GE) and non-gustatory epithelium (NGE), and between GE and the underlying mesenchyme (GM) were enriched in multiple GO terms and KEGG pathways, including many biological processes. Well-known genes for taste sensation were highly expressed in the GE. Moreover, genes of signaling components important in organogenesis (Wnt, TGFβ/ BMP, FGF, Notch, SHH, Erbb) were differentially expressed between GE and GM. Combined with other features of chicken taste buds, e.g., uniquely patterned array and short turnover cycle, our data suggest that chicken gustatory tissue provides an ideal system for multidisciplinary studies, including organogenesis and regenerative medicine.
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影响因子：
4.4
作者：
Coble DJ;Fleming D;Persia ME;Ashwell CM;Rothschild MF;Schmidt CJ;Lamont SJ
通讯作者：
Lamont SJ
影响因子：
6
作者：
Deusch S;Tilocca B;Camarinha-Silva A;Seifert J
通讯作者：
Seifert J
影响因子：
1.5
作者：
Gaillard, Dany;Barlow, Linda A.
通讯作者：
Barlow, Linda A.
影响因子：
2.9
作者：
de Verdal H;Narcy A;Bastianelli D;Chapuis H;Même N;Urvoix S;Le Bihan-Duval E;Mignon-Grasteau S
通讯作者：
Mignon-Grasteau S
DOI：
10.1073/pnas.0607399104
发表时间：
2007-02-13
影响因子：
11.1
作者：
Iwatsuki, Ken;Liu, Hong-Xiang;Margolskee, Robert F.
通讯作者：
Margolskee, Robert F.