Plant DNA Repair and Agrobacterium T-DNA Integration.
Plant DNA Repair and Agrobacterium T-DNA Integration.
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DOI：
10.3390/ijms22168458
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发表时间：
2021-08-06
影响因子：
5.6
通讯作者：
Gelvin SB
中科院分区：
文献类型：
--
作者：
Gelvin SB
农杆菌将DNA （T - DNA）转移到植物细胞中，并整合到植物染色体中。整合的过程被认为涉及T-DNA的入侵和连接，或其复制，进入宿主基因组的缺口或断裂。整合的T-DNA通常在其与植物DNA的连接处含有T-DNA或植物DNA、填充DNA的缺失，以及T-DNA与植物DNA预整合位点之间的微同源性。T - DNA整合也经常与植物基因组重排有关，包括倒置和易位。这些特征与DNA断裂修复后经常发现的特征相似，因此DNA修复机制经常被用来解释T - DNA整合的机制。然而，特定植物DNA修复蛋白和农杆菌蛋白在整合中的参与仍然存在争议，文献中报道了许多相互矛盾的结果。在这篇综述中，我讨论了这些文献，并对其中的许多研究进行了评论。我的结论是，要么多种已知的DNA修复途径可以用于整合，要么一定存在一些未知的途径来促进T - DNA整合到植物基因组中。
Agrobacterium species transfer DNA (T−DNA) to plant cells where it may integrate into plant chromosomes. The process of integration is thought to involve invasion and ligation of T-DNA, or its copying, into nicks or breaks in the host genome. Integrated T−DNA often contains, at its junctions with plant DNA, deletions of T−DNA or plant DNA, filler DNA, and/or microhomology between T-DNA and plant DNA pre-integration sites. T−DNA integration is also often associated with major plant genome rearrangements, including inversions and translocations. These characteristics are similar to those often found after repair of DNA breaks, and thus DNA repair mechanisms have frequently been invoked to explain the mechanism of T−DNA integration. However, the involvement of specific plant DNA repair proteins and Agrobacterium proteins in integration remains controversial, with numerous contradictory results reported in the literature. In this review I discuss this literature and comment on many of these studies. I conclude that either multiple known DNA repair pathways can be used for integration, or that some yet unknown pathway must exist to facilitate T−DNA integration into the plant genome.
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影响因子：
19
作者：
Ceccaldi R;Rondinelli B;D'Andrea AD
通讯作者：
D'Andrea AD
影响因子：
4.3
作者：
Curtis MJ;Belcram K;Bollmann SR;Tominey CM;Hoffman PD;Mercier R;Hays JB
通讯作者：
Hays JB
影响因子：
11.4
作者：
AMBROS, PF;MATZKE, AJM;MATZKE, MA
通讯作者：
MATZKE, MA
影响因子：
3.5
作者：
Bravo-Angel, AM;Hohn, B;Tinland, B
通讯作者：
Tinland, B
DOI：
10.1073/pnas.86.23.9154
发表时间：
1989-12-01
影响因子：
11.1
作者：
DURRENBERGER, F;CRAMERI, A;KOUKOLIKOVANICOLA, Z
通讯作者：
KOUKOLIKOVANICOLA, Z