Molecular differential diagnosis of renal cell carcinomas by microsatellite analysis.
Molecular differential diagnosis of renal cell carcinomas by microsatellite analysis.
复制标题
通过微卫星分析进行肾细胞癌的分子鉴别诊断。
DOI：
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发表时间：
1996
影响因子：
6
通讯作者：
G. Kovacs
中科院分区：
文献类型：
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作者：
P. Bugert;G. Kovacs
近年来，分子细胞遗传学技术的应用导致了肾细胞肿瘤的一种新的遗传分类。新诊断概念的关键方面反映在生物学上不同的实体上，每个实体都以遗传变化的特定组合为特征。为了制定诊断/预后方法，我们基于聚合酶链反应，应用多态性微卫星标记对82例肿瘤标本进行了快速分析。我们将结果与先前评估的细胞遗传学和组织学数据进行了比较。所有非乳头状和嫌色性肾细胞癌（约占所有恶性肾细胞瘤的90%）和一部分肾嗜癌细胞瘤都可以通过检测染色体1、2和3p位点的杂合性缺失得到正确诊断。染色体8p、9p和14q区域的等位基因缺失与非乳头状肾细胞癌的晚期病理阶段相关。除了1号和2号染色体外，6号、10号、13号、17号和21号染色体的杂合性缺失证实了肾细胞肿瘤的嫌色性诊断。使用这种方法，肾细胞肿瘤的鉴别诊断可在1 - 2天内进行。
Recent application of molecular cytogenetic techniques has resulted in a new type of genetic classification of renal cell tumors. The key aspect of the novel diagnostic concept is reflected by biologically distinct entities, each characterized by a specific combination of genetic changes. To work out a diagnostic/prognostic approach, we have applied polymorphic microsatellite markers for a quick analysis, based on polymerase chain reaction, of 82 tumor specimens. We compared the results to previously evaluated cytogenetic and histological data. All nonpapillary and chromophobe renal cell carcinomas, which make up approximately 90% of all malignant renal cell tumors, and a subset of renal oncocytomas were correctly diagnosed by detection of loss of heterozygosity at chromosomal sites 1, 2, and 3p. Allelic losses at chromosomal regions 8p, 9p, and 14q are associated with an advanced pathological stage of nonpapillary renal cell carcinomas. A loss of heterozygosity at chromosomes 6, 10, 13, 17, and 21, in addition to those at chromosomes 1 and 2, confirm the diagnosis of chromophobe renal cell tumors. Using this approach, the differential diagnosis of renal cell tumors could be carried out within 1 or 2 days.
1 号或 14 号染色体杂合性缺失定义了晚期神经母细胞瘤的亚型。
影响因子：
11.2
作者：
Fong,CT;White,PS;Peterson,K;Sapienza,C;Cavenee,WK;Kern,SE;Vogelstein,B;Cantor,AB;Look,AT;Brodeur,GM
通讯作者：
Brodeur,GM
DOI：
10.1073/pnas.89.21.10557
发表时间：
1992-11-01
影响因子：
11.1
作者：
FOUNTAIN, JW;KARAYIORGOU, M;DRACOPOLI, NC
通讯作者：
DRACOPOLI, NC
通过使用黑色素瘤中的纯合缺失来定位推定的肿瘤抑制基因。
DOI：
10.1073/pnas.91.16.7563
发表时间：
1994
影响因子：
11.1
作者：
Weaver-Feldhaus,J;Gruis,NA;Neuhausen,S;LePaslier,D;Stockert,E;Skolnick,MH;Kamb,A
通讯作者：
Kamb,A