Low-temperature-specific effects of PHYTOCHROME C on the circadian clock in Arabidopsis suggest that PHYC underlies natural variation in biological timing
Low-temperature-specific effects of PHYTOCHROME C on the circadian clock in Arabidopsis suggest that PHYC underlies natural variation in biological timing
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PHYTOCHROME C 对拟南芥生物钟的低温特异性影响表明 PHYC 是生物计时自然变异的基础
DOI：
10.1101/030577
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Edwards K
中科院分区：
文献类型：
--
作者：
Edwards K
生物钟是真核生物和某些原核生物基因调控和细胞生理的基本特征，也是系统生物学中典型的基因调控网络。拟南芥的昼夜节律系统是复杂的，部分原因在于其光传导途径。拟南芥属物种佛得角群岛（Cape Verde Islands）（Cvi-0）和兰茨贝格直立拟南芥（Landsberg erecta）（Ler）之间的天然遗传变异的分析鉴定了染色体V上的主要的温度特异性数量性状基因座（QTL），其改变了叶运动的昼夜节律周期（Edwards等人，Genetics，2005）。我们测试了近等基因系（NILs），以证实该PerCv 5cQTL上的Ler等位基因在12°C下延长了昼夜节律周期，在较高温度下几乎没有影响。植物色素C基因位于QTL区间内，含有多个序列变异。除了在27°C下外，在白色光下，携带T-DNA插入到PHYC或PHYC缺失的植物也延长了昼夜节律周期。这些结果扩展了最近显示PhyC在红光中的作用的数据，证实了植物昼夜节律系统中涉及的感光蛋白的数量为11。加强了光输入机制和温度对时钟的影响之间的联系。PHYC内的自然遗传变异可能是PerCv 5cQTL的基础。我们的研究结果表明，thPHYC-Ler单倍型组内的功能变异可能有助于昼夜节律系统的进化，并可能与时钟相关的表型，如开花时间。这些结果之前已经通过了同行评审，因此我们在此提供了可引用的预印本。
The circadian clock is a fundamental feature of gene regulation and cell physiology in eukaryotes and some prokaryotes, and an exemplar gene regulatory network in Systems Biology. The circadian system inArabidopsis thalianais complex in part due to its photo-transduction pathways. Analysis of natural genetic variation between Arabidopsis accessions Cape Verde Islands (Cvi-0) and Landsberg erecta (Ler) identified a major, temperature-specific Quantitative Trait Locus (QTL) on chromosome V that altered the circadian period of leaf movement (Edwards et al., Genetics, 2005). We tested Near-Isogenic Lines (NILs) to confirm that Leralleles at thisPerCv5cQTL lengthened the circadian period at 12°C, with little effect at higher temperatures. ThePHYTOCHROME Cgene lies within the QTL interval, and contains multiple sequence variants. Plants carrying either a T-DNA-insertion intoPHYCor a deletion ofPHYCalso lengthened circadian period under white light, except at 27°C.phyBandphyABEmutants lengthened period only at 12°C. These results extend recent data showing PhyC effects in red light, confirming the number of photoreceptor proteins implicated in the plant circadian system at eleven. The connection between light input mechanisms and temperature effects on the clock is reinforced. Natural genetic variation withinPHYCis likely to underlie thePerCv5cQTL. Our results suggest that functional variation within thePHYC-Lerhaplotype group might contribute to the evolution of the circadian system and possibly to clock-related phenotypes such as flowering time. These results have previously passed peer-review, so we provide them in this citable preprint.
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影响因子：
11.6
作者：
Baudry, Antoine;Ito, Shogo;Imaizumi, Takato
通讯作者：
Imaizumi, Takato
DOI：
10.1073/pnas.1221738110
发表时间：
2013-01-22
影响因子：
11.1
作者：
Hu, Wei;Franklin, Keara A.;Lagarias, J. Clark
通讯作者：
Lagarias, J. Clark
影响因子：
3.3
作者：
Colot, HV;Loros, JJ;Dunlap, JC
通讯作者：
Dunlap, JC
影响因子：
3.3
作者：
Pankin A;Campoli C;Dong X;Kilian B;Sharma R;Himmelbach A;Saini R;Davis SJ;Stein N;Schneeberger K;von Korff M
通讯作者：
von Korff M
影响因子：
9.9
作者：
Seaton DD;Smith RW;Song YH;MacGregor DR;Stewart K;Steel G;Foreman J;Penfield S;Imaizumi T;Millar AJ;Halliday KJ
通讯作者：
Halliday KJ