Meis2 Is Required for Inner Ear Formation and Proper Morphogenesis of the Cochlea.
Meis2 Is Required for Inner Ear Formation and Proper Morphogenesis of the Cochlea.
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DOI：
10.3389/fcell.2021.679325
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发表时间：
2021
影响因子：
5.5
通讯作者：
Schimmang T
中科院分区：
文献类型：
--
作者：
Durán Alonso MB;Vendrell V;López-Hernández I;Alonso MT;Martin DM;Giráldez F;Carramolino L;Giovinazzo G;Vázquez E;Torres M;Schimmang T
Meis基因已被证明控制中枢和外周神经系统发育过程中的重要过程。在这里，我们探讨了Meis2基因在脊椎动物内耳诱导和耳蜗形成过程中的作用。Meis2在内耳诱导所需的几种组织和耳蜗管的非感觉组织中表达。Meis2在小鼠中的整体失活导致耳泡的尺寸严重减小。Meis2的组织特异性敲除揭示其在后脑中的表达对于耳泡形成是必不可少的。Meis2在内耳中的失活本身导致耳蜗管的异常卷曲。通过分析从Meis2突变体获得的转录组和耳细胞系的ChIPseq分析，我们定义了可能直接或间接参与耳蜗形态发生的Meis2的候选靶基因。总之，这些数据表明Meis 2对于内耳形成是必不可少的，并提供了一个切入点来揭示耳蜗管正确卷曲的网络。
Meis genes have been shown to control essential processes during development of the central and peripheral nervous system. Here we have explored the roles of the Meis2 gene during vertebrate inner ear induction and the formation of the cochlea. Meis2 is expressed in several tissues required for inner ear induction and in non-sensory tissue of the cochlear duct. Global inactivation of Meis2 in the mouse leads to a severely reduced size of the otic vesicle. Tissue-specific knock outs of Meis2 reveal that its expression in the hindbrain is essential for otic vesicle formation. Inactivation of Meis2 in the inner ear itself leads to an aberrant coiling of the cochlear duct. By analyzing transcriptomes obtained from Meis2 mutants and ChIPseq analysis of an otic cell line, we define candidate target genes for Meis2 which may be directly or indirectly involved in cochlear morphogenesis. Taken together, these data show that Meis2 is essential for inner ear formation and provide an entry point to unveil the network underlying proper coiling of the cochlear duct.
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