Immunological and anti-oxidant profiles of malarial children in Abuja, Nigeria.
Immunological and anti-oxidant profiles of malarial children in Abuja, Nigeria.
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DOI：
10.37796/2211-8039.1010
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bakare M
中科院分区：
文献类型：
--
作者：
Abdullahi IN;Musa S;Emeribe AU;Muhammed M;Mustapha JO;Shuwa HA;Haruna S;Abubakar SD;Billyrose OMA;Bakare M
疟疾流行区儿童恶性疟的临床症状、细胞免疫反应和血清细胞因子的动态平衡与疟原虫密度有关。本研究的目的是评估CD 4+和CD 8 + T细胞，白细胞亚群，IL-6，IL-10和氧化应激的生物标志物感染不同级别的疟疾参加阿布贾大学教学医院和国立医院，阿布贾，尼日利亚的儿童。这项病例对照研究涉及165名儿童（5至12岁）的血液样本。其中包括45名轻度疟疾儿童，40名中度、重度疟疾儿童和明显健康的儿童（对照组）。采用酶联免疫吸附试验（ELISA）测定血清细胞因子、铁蛋白、丙二醛（MDA）、抗坏血酸、α-生育酚水平。分别用全自动血细胞分析仪和流式细胞仪计数白细胞分类和CD 4 +/CD 8 + T细胞计数。所有患疟疾的儿童只有恶性疟原虫。轻度疟疾儿童的男女比例为1.5：1（平均值± SD年龄为8.5 ± 1.9岁）。然而，其他组的男女比例为1：1，中度、重度疟疾儿童和对照组的平均年龄分别为9.2 ± 2.3、9.8 ± 2.2、8.5 ± 1.5。中性粒细胞和单核细胞与疟虫血症3个级别呈正相关，但无统计学意义（P>0.05）。严重疟疾与淋巴细胞计数之间存在显著负相关（p = 0.048; r =-0.647）。嗜酸性粒细胞与中度疟疾（p = 0.03; r = 0.994）和重度疟疾（p = 0.006; r = 0.825）呈正相关。随着疟疾密度的增加，血清抗坏血酸显著下降（p<0.0001）。然而，4组儿童的血清α-生育酚浓度无差异（p = 0.182）。血清铁蛋白和MDA随疟疾密度的增加而显著升高（p<0.0001）。随着疟疾密度的增加，CD 4 + T和CD 8 + T细胞计数显著下降（p<0.0001）。血清IL-10和IL-6水平随疟疾密度的增加而显著升高（P<0.0001）。基于这些发现，重症疟疾与CD 4+和CD 8 + T细胞计数下降、IL-6上调和氧化应激生物标志物血清水平升高显著相关。
The clinical symptoms, cellular immune response, and serum cytokine homeostasis during falciparum malaria among children living in endemic regions depend on the parasite densities. This study aims to evaluate the CD4+ and CD8+ T cells, leucocytes subpopulations, IL-6, IL-10 and biomarkers of oxidative stress among children infected with varying grades of malaria attending the University of Abuja Teaching Hospital and National Hospital, Abuja, Nigeria. This case-control study involved blood samples collected from 165 children (between 5 and 12 years). This comprised 45 children with mild malaria, 40 each with moderate, severe malaria and apparently healthy (control). Serum cytokines, ferritin, malonaldehyde (MDA), ascorbate, α-tocopherol levels were determined by Enzyme-Linked ImmunoSorbent Assay (ELISA). Leucocytes differentials and CD4+/CD8+ T cells counts were enumerated by automated hematology analyzer and flow cytometry, respectively. All malarial children had only Plasmodium falciparum. The male to female ratio of children with mild malaria was 1.5:1 (mean ± SD age of 8.5 ± 1.9 years). However, other groups had 1:1 male to female ratio and mean ages of 9.2 ± 2.3, 9.8 ± 2.2, 8.5 ± 1.5 for children with moderate, severe malaria and control, respectively. There was a positive but not significant association of neutrophils and monocytes with the 3 grades of malaria parasitemia (p>0.05). There was a negative and significant correlation between severe malaria and lymphocyte count (p = 0.048; r = −0.647). However, there was positive and significant correlation between eosinophil with moderate (p = 0.03; r = 0.994) and severe malaria (p = 0.006; r = 0.825). There was a significant decline in serum ascorbate with increased malaria density (p<0.0001). However, there was no difference in the serum α-tocopherol concentration within the 4 groups of children (p = 0.182). Serum ferritin and MDA significantly elevated with an increase in malaria density (p<0.0001). There was a significant decline in CD4+ T and CD8+ T cells counts with an increase in malaria densities (p<0.0001). Serum IL-10 and IL-6 significantly elevated with increased malaria density (p<0.0001). Based on these findings, severe malaria was significantly associated with declined CD4+ and CD8+ T cell counts, upregulation of IL-6, and high serum levels of oxidative stress biomarkers.
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DOI：
10.1128/cvi.00533-16
发表时间：
2017-04
期刊：
Clinical and vaccine immunology : CVI
影响因子：
--
作者：
Mandala WL;Msefula CL;Gondwe EN;Drayson MT;Molyneux ME;MacLennan CA
通讯作者：
MacLennan CA
影响因子：
3.3
作者：
Efunshile, Aldnwale Michael;Olawale, Temitope;Konig, Brigitte
通讯作者：
Konig, Brigitte
DOI：
10.5539/gjhs.v4n4p139
发表时间：
2012-06-15
期刊：
Global journal of health science
影响因子：
--
作者：
M, Eze Evelyn;Ezeiruaku, F C;Ukaji, D C
通讯作者：
Ukaji, D C
影响因子：
5.7
作者：
Dunst J;Kamena F;Matuschewski K
通讯作者：
Matuschewski K
影响因子：
3
作者：
Frimpong, Augustina;Kusi, Kwadwo Asamoah;Ndifon, Wilfred
通讯作者：
Ndifon, Wilfred