Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.
Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.
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通过荧光原位杂交与复合全染色体特异性 DNA 探针的三重组合来分析辐射诱导的染色体畸变。
DOI：
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发表时间：
1992
影响因子：
2.6
通讯作者：
M. Bauchinger
中科院分区：
文献类型：
--
作者：
E. Schmid;H. Zitzelsberger;H. Braselmann;J. Gray;M. Bauchinger
应用荧光原位杂交(FISH)技术，结合人染色体1、4和12号染色体的三个复合全染色体特异性DNA探针，分析了体外辐射诱导的外周血淋巴细胞的双着丝粒和对称性易位。FISH可以快速有效地检测到易位。在给定剂量下，它们的频率是双着丝粒频率的1.8倍。易位和双着丝粒的剂量-反应曲线呈线性二次曲线，易位的二次分量明显较高。讨论了FISH在辐射照射生物剂量学中评价稳定易位的应用。
Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.
DOI：
10.1073/pnas.83.9.2934
发表时间：
1986-05-01
影响因子：
11.1
作者：
PINKEL, D;STRAUME, T;GRAY, JW
通讯作者：
GRAY, JW
DOI：
10.1073/pnas.85.23.9138
发表时间：
1988-12-01
影响因子：
11.1
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通讯作者：
GRAY, J