Ageing and the Diurnal Expression of mRNAs for Vasoactive Intestinal Peptide and for the VPAC2 and PAC1 Receptors in the Suprachiasmatic Nucleus of Male Rats
Ageing and the Diurnal Expression of mRNAs for Vasoactive Intestinal Peptide and for the VPAC2 and PAC1 Receptors in the Suprachiasmatic Nucleus of Male Rats
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雄性大鼠视交叉上核中血管活性肠肽和 VPAC2 和 PAC1 受体的衰老和 mRNA 的昼夜表达
DOI：
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发表时间：
2004
影响因子：
3.2
通讯作者：
C. Coen
中科院分区：
文献类型：
--
作者：
I. Kalló;T. Kalamatianos;H. Piggins;C. Coen
衰老改变了哺乳动物昼夜节律的基本方面;其影响包括节律幅度降低、周期长度改变和对明/暗周期的夹带。这种变化可能反映了视交叉上核（SCN）内细胞功能的中断，SCN是主要的昼夜节律起搏器的部位。在SCN中，合成血管活性肠肽（VIP）的神经元接受各种输入，包括含有垂体腺苷酸环化酶激活肽（PACAP）的视网膜下丘脑投射。SCN VIP细胞与局部神经元建立联系，并将传出神经送出细胞核。相当多的证据表明VIP和PACAP在昼夜节律的维持和/或夹带光Zeitgebers。这些作用涉及一个不同的受体家族的成员;两个这样的受体，VPAC 2和PAC 1的mRNA存在于SCN中。本研究采用同位素原位杂交技术研究了12：12 h光/暗周期下雄性大鼠SCN中VIP、VPAC 2和PAC 1 mRNA表达的衰老效应。 在光/暗周期的8个时间点，对年轻成年（2-3个月）或老年（19-20个月）大鼠的电影放射自显影图进行分析，结果显示老年组的昼夜节律丧失，VIP mRNA水平降低。 两组大鼠的VPAC 2受体mRNA均存在昼夜节律，但老年大鼠的VPAC 2受体mRNA水平降低。PAC 1受体mRNA表达在两组间无差异。本研究结果表明，老龄化减少VIP和VPAC 2受体mRNA和消除昼夜表达的VIP mRNA内的SCN的老年雄性大鼠。
Ageing alters fundamental aspects of circadian rhythmicity in mammals; the effects include reduced rhythm amplitude and alterations in period length and in entrainment to the light/dark cycle. Such changes may reflect disruptions in cellular function within the suprachiasmatic nucleus (SCN), the site of the predominant circadian pacemaker. In the SCN, vasoactive intestinal peptide (VIP)‐synthesizing neurones receive various inputs, including retinohypothalamic projections containing pituitary adenylate cyclase activating peptide (PACAP). SCN VIP cells establish connections with local neurones and send efferents beyond the nucleus. Considerable evidence implicates VIP and PACAP in circadian rhythm maintenance and/or entrainment to photic Zeitgebers. These actions involve members of a distinct family of receptors; mRNAs for two such receptors, VPAC2 and PAC1, are present in the SCN. This study used isotopic in situ hybridization to examine the effects of ageing on expression of mRNAs for VIP, VPAC2 and PAC1 in the SCN of male rats under a 12 : 12 h light/dark cycle. Analysis of film autoradiographs from young adult (2–3 months) or aged (19–20 months) rats, at eight time points across the light/dark cycle, showed loss of diurnal rhythmicity and reduced levels for VIP mRNA in the aged group. A diurnal rhythm of VPAC2 receptor mRNA was present in both groups, but its levels were reduced in the aged rats. There were no differences between the two groups for PAC1 receptor mRNA expression. The present results indicate that ageing reduces VIP and VPAC2 receptor mRNA and eliminates diurnal expression of VIP mRNA within the SCN of aged male rats.
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影响因子：
21.1
作者：
A. Harmar;A. Arimura;I. Gozes;L. Journot;M. Laburthe;J. Pisegna;S. Rawlings;P. Robberecht;S. Said;S. Sreedharan;S. Wank;J. Waschek
通讯作者：
A. Harmar;A. Arimura;I. Gozes;L. Journot;M. Laburthe;J. Pisegna;S. Rawlings;P. Robberecht;S. Said;S. Sreedharan;S. Wank;J. Waschek
年老大鼠的视交叉上核是否像年轻大鼠那样振荡？
DOI：
10.1152/ajpregu.1993.265.5.r1216
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Satinoff,E;Li,H;Tcheng,TK;Liu,C;McArthur,AJ;Medanic,M;Gillette,MU
通讯作者：
Gillette,MU
将胎儿视交叉上核移植到中年大鼠体内可恢复宿主体内的昼夜 Fos 表达。
DOI：
10.1152/ajpregu.1997.272.1.r422
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
Cai,A;Lehman,MN;Lloyd,JM;Wise,PM
通讯作者：
Wise,PM
影响因子：
4.8
作者：
R. Hauger;K. Thrivikraman;P. Plotsky
通讯作者：
R. Hauger;K. Thrivikraman;P. Plotsky
单肽神经递质反义拮抗作用对生物钟的急性影响。
DOI：
10.1152/ajpregu.1996.270.1.r283
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Scarbrough,K;Harney,JP;Rosewell,KL;Wise,PM
通讯作者：
Wise,PM