Novel drug delivery system based on docetaxel-loaded nanocapsules as a therapeutic strategy against breast cancer cells.
Novel drug delivery system based on docetaxel-loaded nanocapsules as a therapeutic strategy against breast cancer cells.
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DOI：
10.3390/ijms13044906
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发表时间：
2012
影响因子：
5.6
通讯作者：
Aránega A
中科院分区：
文献类型：
--
作者：
Sánchez-Moreno P;Boulaiz H;Ortega-Vinuesa JL;Peula-García JM;Aránega A
在癌症治疗领域，基于核壳结构的脂质纳米胶囊是用于输送疏水性药物（如多西紫杉醇）的有前途的载体。这项工作的主要目的是评估负载多西紫杉醇的脂质纳米胶囊是否提高了游离多西紫杉醇在乳腺癌细胞中的抗肿瘤作用。采用溶剂置换法合成了三种多西紫杉醇脂质纳米胶囊。在经磺胺罗丹明 B 比色法处理的乳腺癌 (MCF-7) 细胞中评估细胞毒性测定。通过流式细胞术和膜联蛋白 V-FITC 研究细胞周期，并通过碘化丙啶测定评估细胞凋亡。当药物被封装在脂质纳米颗粒中时，多西他赛的抗增殖作用比游离时更早出现。负载多西紫杉醇的脂质纳米胶囊显着增强了IC50率的降低，并且处理后的细胞出现细胞凋亡和细胞周期从G(1)期提前进展至G(2)-M期。游离多西紫杉醇对乳腺癌细胞的化疗效果通过将其封装在脂质纳米胶囊中而得到改善。这种方法有可能克服传统化疗的一些主要局限性，并且可能是未来乳腺癌治疗应用的一种有前途的策略。
In the field of cancer therapy, lipid nanocapsules based on a core-shell structure are promising vehicles for the delivery of hydrophobic drugs such as docetaxel. The main aim of this work was to evaluate whether docetaxel-loaded lipid nanocapsules improved the anti-tumor effect of free docetaxel in breast cancer cells. Three docetaxel-loaded lipid nanocapsules were synthesized by solvent displacement method. Cytotoxic assays were evaluated in breast carcinoma (MCF-7) cells treated by the sulforhodamine B colorimetric method. Cell cycle was studied by flow cytometry and Annexin V-FITC, and apoptosis was evaluated by using propidium iodide assays. The anti-proliferative effect of docetaxel appeared much earlier when the drug was encapsulated in lipid nanoparticles than when it was free. Docetaxel-loaded lipid nanocapsules significantly enhanced the decrease in IC50 rate, and the treated cells evidenced apoptosis and a premature progression of the cell cycle from G(1) to G(2)-M phase. The chemotherapeutic effect of free docetaxel on breast cancer cells is improved by its encapsulation in lipid nanocapsules. This approach has the potential to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in breast cancer therapy.
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影响因子：
8
作者：
Hernandez-Vargas, H.;Palacios, J.;Moreno-Bueno, G.
通讯作者：
Moreno-Bueno, G.
影响因子：
10.8
作者：
Immordino, ML;Brusa, P;Cattel, L
通讯作者：
Cattel, L
影响因子：
5.8
作者：
Liu, Donghua;Liu, Zhihong;Zhang, Na
通讯作者：
Zhang, Na
影响因子：
3.7
作者：
Qin YY;Li H;Guo XJ;Ye XF;Wei X;Zhou YH;Zhang XJ;Wang C;Qian W;Lu J;He J
通讯作者：
He J
影响因子：
16.1
作者：
Florence, AT;Hussain, N
通讯作者：
Hussain, N