Calcium imaging of infrared-stimulated activity in rodent brain.
Calcium imaging of infrared-stimulated activity in rodent brain.
复制标题
DOI：
10.1016/j.ceca.2014.01.004
复制
发表时间：
2014-04
期刊：
影响因子：
4
通讯作者：
Mahadevan-Jansen A
中科院分区：
文献类型：
--
作者：
Cayce JM;Bouchard MB;Chernov MM;Chen BR;Grosberg LE;Jansen ED;Hillman EM;Mahadevan-Jansen A
红外神经刺激（INS）是一种很有前途的神经刺激技术，它可以以高空间精度激活神经组织，而不需要外源性药物。然而，关于红外光如何在细胞水平上与神经组织相互作用，特别是在活体大脑中，人们知之甚少。在本研究中，我们使用钙敏感染料成像在宏观和微观尺度上探索INS对皮质钙动力学的时空效应。观察到的INS诱发的钙信号表现出快和慢的成分，表明多种细胞机制的激活。慢成分的诱发信号表现出类似波的性质，表明网络激活，并通过药理学和双光子成像验证来自星形胶质细胞。我们还提供了证据表明，快速钙信号可能已经通过谷氨酸瞬变的调制诱发。这项研究表明，脉冲红外光可以诱导星形胶质细胞和神经元的细胞内钙调节，提供了新的见解INS在大脑中的作用机制。
Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.
登录
查看更多内容
影响因子：
3.4
作者：
Oliver, AE;Baker, GA;Crowe, JH
通讯作者：
Crowe, JH
影响因子：
3.6
作者：
Radosevich, Andrew J.;Bouchard, Matthew B.;Burgess, Sean A.;Chen, Brenda R.;Hillman, Elizabeth M. C.
通讯作者：
Hillman, Elizabeth M. C.
影响因子：
1.9
作者：
HALE, GM;QUERRY, MR
通讯作者：
QUERRY, MR
影响因子：
48
作者：
Schulz, Kristina;Sydekum, Esther;Helmchen, Fritjof
通讯作者：
Helmchen, Fritjof
影响因子：
11
作者：
Kahn, Elyne;D'Haese, Pierre-Francois;Konrad, Peter
通讯作者：
Konrad, Peter