Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction.
Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction.
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使用渗透泵局部连续释放胶质细胞源性神经营养因子促进海绵体神经损伤引起的勃起功能障碍动物模型的副交感神经康复
DOI：
10.21037/tau-20-1110
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发表时间：
2021-01
影响因子：
2
通讯作者：
Peng J
中科院分区：
文献类型：
--
作者：
Li H;Zhang Z;Fang D;Tang Y;Peng J
背景 神经损伤相关的勃起功能障碍 (ED) 是传统 ED 治疗效果不佳的类型之一。我们之前的实验已经证明干细胞或其他治疗方式旁分泌各种神经营养因子（NTF）是神经损伤相关 ED 恢复的潜在机制。胶质细胞源性神经营养因子（GDNF）是神经纤维尤其是副交感神经再生所必需的NTF之一。本研究的目的是探讨局部连续给予 GDNF 是否有利于神经损伤引起的 ED 的功能和组织学恢复。方法本研究使用八周龄雄性斯普拉格-道利大鼠。将大鼠随机分为 5 组：假手术（Sham）、双侧海绵神经损伤（BCNI）和安慰剂治疗、BCNI 和 0.1 µg/100 µL GDNF 治疗（BCNI+GDNF 0.1）、BCNI 和 1 µg/100 µL GDNF 治疗（BCNI+GDNF 1）、BCNI 和 10 µg/100 µL GDNF 治疗（BCNI+GDNF 10）。 GDNF 使用渗透泵技术进行给药，该技术可在局部连续输送 GDNF 28 天，无需外部连接或频繁处理动物。检查并比较治疗后各组的性功能恢复情况、神经纤维再生情况以及神经营养受体的表达情况。结果局部连续GDNF释放治疗以剂量依赖性方式增加了性行为测试中的平均插入次数和勃起功能测试中的海绵体内压（ICP）。渗透泵植入引起局部 GDNF 浓度增加和轻度炎症反应。 GDNF 部分促进了主要骨盆神经节 (MPG) 中 GDNF 受体的基因表达和沿尿道的神经再生。这些变化与 GDNF 治疗组中神经纤维的增加有关，尤其是阴茎背神经 (DNP) 中的副交感神经纤维。结论 总之，我们的项目说明了局部连续 GDNF 给药对于神经损伤引起的 ED 功能和组织学恢复的良好效果。
Background Nerve injury-related erectile dysfunction (ED) is one of the types that respond poorly to conventional ED treatments. Our previous experiments have demonstrated the paracrine of various neurotrophic factors (NTFs) by stem cells or other treatment modalities as a potential mechanism in the recovery of nerve injury-related ED. Glial cell derived neurotrophic factor (GDNF) is one of the essential NTFs for the regeneration of nerve fibers, especially for parasympathetic nerves. The aim of this study is to explore if local continuous GDNF administration is beneficial for the functional and histological recovery of nerve injury induced ED. Methods Eight-week-old male Sprague-Dawley rats were used for this study. Rats were randomly grouped into 5: Sham surgery (Sham), bilateral cavernous nerve injury (BCNI) and placebo treatment, BCNI and 0.1 µg/100 µL GDNF treatment (BCNI+GDNF 0.1), BCNI and 1 µg/100 µL GDNF treatment (BCNI+GDNF 1), BCNI and 10 µg/100 µL GDNF treatment (BCNI+GDNF 10). GDNF was administered using an osmotic pump technique which would deliver GDNF locally and continuously for 28 days without the need for external connections or frequent handling of animals. Recovery of sexual function, nerve fibers regeneration, and expression of neurotrophic receptors were examined and compared among groups after the treatment. Results Local continuous GDNF release treatment increased the average number of intromissions in the sexual behavior test and intracavernous pressure (ICP) in the erectile function test in a dose dependent manner. Osmotic pump implantation induced increased local GDNF concentration and mild inflammatory response. Gene expression of GDNF receptors in major pelvic ganglion (MPG) and nerve regeneration along the urethra were partially promoted by GDNF. These changes were associated with increased nerve fibers especially the parasympathetic nerve fibers in dorsal nerve of penis (DNP) in GDNF treated groups. Conclusions In conclusion, our project illustrated the promising effects of local continuous GDNF administration for the functional and histological recovery of nerve injury-induced ED.
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影响因子：
4.2
作者：
Hannan, Johanna L.;Albersen, Maarten;Stopak, Bernard L.;Liu, Xiaopu;Burnett, Arthur L.;Hoke, Ahmet;Bivalacqua, Trinity J.
通讯作者：
Bivalacqua, Trinity J.
影响因子：
2.6
作者：
May, Florian;Buchner, Alexander;Matiasek, Kaspar
通讯作者：
Matiasek, Kaspar
DOI：
10.1186/1749-7221-2-5
发表时间：
2007-12-01
影响因子：
0.7
作者：
Bella, Anthony J.;Fandel, Thomas M.;Lue, Tom F.
通讯作者：
Lue, Tom F.
影响因子：
--
作者：
Mas, M
通讯作者：
Mas, M
影响因子：
2.9
作者：
Forrest SL;Payne SC;Keast JR;Osborne PB
通讯作者：
Osborne PB