Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly.
Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly.
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DOI：
10.1016/j.cell.2008.06.018
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发表时间：
2008-07-25
期刊：
影响因子：
64.5
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li Q;Zhou H;Wurtele H;Davies B;Horazdovsky B;Verreault A;Zhang Z
染色质组装因子1(CAF-1)和Rtt106参与了新合成的组蛋白在复制DNA上的沉积形成核小体。这一过程对于维持基因组的稳定性和在增殖细胞中遗传功能特化的染色质结构是至关重要的。然而，在DNA复制偶联的核小体组装途径中，新合成组蛋白的乙酰化的分子功能仍然是个谜。在这里，我们证明了组蛋白H3在赖氨酸56位乙酰化(H3K56Ac)被结合到复制的DNA上，并通过增加Caf-1和Rtt106与组蛋白H3的结合亲和力，H3K56Ac增强了这些组蛋白伴侣蛋白将DNA组装到核小体的能力。遗传分析表明，H3K56Ac在核小体组装中与H3和H4的N末端残基的乙酰化作用是非冗余的。这些结果揭示了H3K56Ac调控CAF-1和Rtt106介导的复制偶联核小体组装的机制。
Chromatin assembly factor 1 (CAF-1) and Rtt106 participate in the deposition of newly synthesized histones onto replicating DNA to form nucleosomes. This process is critical for the maintenance of genome stability and inheritance of functionally specialized chromatin structures in proliferating cells. However, the molecular functions of the acetylation of newly synthesized histones in this DNA replication-coupled nucleosome assembly pathway remain enigmatic. Here we show that histone H3 acetylated at lysine 56 (H3K56Ac) is incorporated onto replicating DNA and, by increasing the binding affinity of CAF-1 and Rtt106 for histone H3, H3K56Ac enhances the ability of these histone chaperones to assemble DNA into nucleosomes. Genetic analysis indicates that H3K56Ac acts in a nonredundant manner with the acetylation of the N-terminal residues of H3 and H4 in nucleosome assembly. These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106.
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DOI：
10.1126/science.1135862
发表时间：
2007-02-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Driscoll R;Hudson A;Jackson SP
通讯作者：
Jackson SP
影响因子：
4.8
作者：
Garcia, Benjamin A.;Hake, Sandra B.;Hunt, Donald F.
通讯作者：
Hunt, Donald F.
影响因子：
64.8
作者：
Natsume, Ryo;Eitoku, Masamitsu;Senda, Toshiya
通讯作者：
Senda, Toshiya
影响因子：
16
作者：
Altaf, Mohammed;Utley, Rhea T.;Cote, Jacques
通讯作者：
Cote, Jacques
影响因子：
64.5
作者：
Goldberg, Aaron D.;Allis, C. David;Bernstein, Emily
通讯作者：
Bernstein, Emily