Toxicoproteomic Analysis of Differentially Expressed Proteins in Rat Liver by DEHP
Toxicoproteomic Analysis of Differentially Expressed Proteins in Rat Liver by DEHP
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DEHP 对大鼠肝脏差异表达蛋白的毒物蛋白质组学分析
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Mi
中科院分区：
文献类型：
--
作者：
Bu;Ahreum Seong;Seul;Wan;J. Ryu;Mi
内分泌干扰物邻苯二甲酸二(2-乙基己基)酯(DEHP)是一种增塑剂，用于日常生活中普遍存在的聚氯乙烯产品。DEHP对肝、肾、肺、心脏、生殖器官和内分泌系统有潜在的不良影响。关于DEHP毒性的许多毒理学数据已经发表，但尚未有完整的蛋白质图谱的报道。本研究首次用彗星试验(单细胞凝胶电泳法)检测了DEHP对大鼠淋巴细胞DNA的氧化损伤作用。此外，利用毒物蛋白质组学工具检测了DEHP诱导的大鼠肝脏蛋白质谱的变化。在二维凝胶上，DEHP发现肝脏中的34个蛋白质点被显著地解除了调控。其中，DEHP上调了20个点，下调了14个点。
The endocrine disrupting chemical, di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride products ubiquitous in our daily lives. DEHP has potentially adverse effects on the liver, kidney, lung, heart, reproductive organs and endocrine systems. Many toxicological data on the DEHP toxicity have been stated, but complete protein profiles have not yet been reported. In this study, DEHP-induced oxidative DNA damage in rat lymphocyte was evaluated by Comet assay (single-cell gel electrophoresis) for the first time. Moreover, DEHP-induced protein profile alterations were examined in rat liver by using toxicoproteomic tools. 34 protein spots in the liver were identified to be significantly deregulated by DEHP on the 2-dimensional gel. Among them, 20 spots were up-regulated and 14 spots down-regulated by DEHP.
DOI：
10.1016/s0027-5107(99)00232-8
发表时间：
2000-03-17
影响因子：
2.3
作者：
Corton, JC;Lapinskas, PJ;Gonzalez, FJ
通讯作者：
Gonzalez, FJ