TOMATOMA: a novel tomato mutant database distributing Micro-Tom mutant collections.
TOMATOMA: a novel tomato mutant database distributing Micro-Tom mutant collections.
复制标题
DOI：
10.1093/pcp/pcr004
复制
发表时间：
2011-02
影响因子：
4.9
通讯作者：
Ezura H
中科院分区：
文献类型：
--
作者：
Saito T;Ariizumi T;Okabe Y;Asamizu E;Hiwasa-Tanase K;Fukuda N;Mizoguchi T;Yamazaki Y;Aoki K;Ezura H
番茄是研究浆果型果实的植物和茄科植物实验研究的极好模型，因为它具有保守的遗传组织。本研究以矮小、快速生长的番茄品种Micro-Tom为背景，产生了一个综合性突变体番茄群体。在本研究和前人的研究中，利用甲烷磺酸乙酯（EMS）诱变和γ射线辐照制备了一个包括8,598和6,422个M2诱变系，并对这些M2植株的可见表型变化进行了开发和研究。共检测了9183个独立的M2家族、91830个M2植株的表型变异，分离出1048个个体突变体。随后，将观察到的突变表型分为15大类和48亚类。总体而言，在1048个突变体中发现了1819个表型类别。其中549个为多效性突变体，499个为非多效性突变体。突变文库中每个位点存在多个不同的突变等位基因，表明突变群体接近饱和。回交遗传分析表明，该突变在BC1F2群体中成功遗传，证实了M2植株形态表型的可重复性。为了整合和管理突变体的可见表型和其他相关数据，我们开发了计算机数据库TOMATOMA，这是一个连接突变系名称和表型类别的关系系统接口模块。TOMATOMA是一个免费访问的数据库，这些突变资源可以通过TOMATOMA （http://tomatoma.nbrp.jp/index.jsp）获得。
The tomato is an excellent model for studies of plants bearing berry-type fruits and for experimental studies of the Solanaceae family of plants due to its conserved genetic organization. In this study, a comprehensive mutant tomato population was generated in the background of Micro-Tom, a dwarf, rapid-growth variety. In this and previous studies, a family including 8,598 and 6,422 M2 mutagenized lines was produced by ethylmethane sulfonate (EMS) mutagenesis and γ-ray irradiation, and this study developed and investigated these M2 plants for alteration of visible phenotypes. A total of 9,183 independent M2 families comprising 91,830 M2 plants were inspected for phenotypic alteration, and 1,048 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 15 major categories and 48 subcategories. Overall, 1,819 phenotypic categories were found in 1,048 mutants. Of these mutants, 549 were pleiotropic, whereas 499 were non-pleiotropic. Multiple different mutant alleles per locus were found in the mutant libraries, suggesting that the mutagenized populations were nearly saturated. Additionally, genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC1F2 populations, confirming the reproducibility in the morphological phenotyping of the M2 plants. To integrate and manage the visible phenotypes of mutants and other associated data, we developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories. TOMATOMA is a freely accessible database, and these mutant recourses are available through the TOMATOMA (http://tomatoma.nbrp.jp/index.jsp).
登录
查看更多内容
影响因子：
5.1
作者：
Gady AL;Hermans FW;Van de Wal MH;van Loo EN;Visser RG;Bachem CW
通讯作者：
Bachem CW
影响因子：
30.8
作者：
Cong, Bin;Barrero, Luz S.;Tanksley, Steven D.
通讯作者：
Tanksley, Steven D.
影响因子：
11.6
作者：
Fulton, TM;Van der Hoeven, R;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
9.4
作者：
Dielen, V;Quinet, M;Kinet, JM
通讯作者：
Kinet, JM
DOI：
10.1155/2007/64358
发表时间：
2007
期刊：
International journal of plant genomics
影响因子：
--
作者：
Foolad MR
通讯作者：
Foolad MR