MSC Functional Phenotype: Assay, Age and Source Dependence
MSC Functional Phenotype: Assay, Age and Source Dependence
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MSC 功能表型：检测、年龄和来源依赖性
DOI：
10.14800/sp.934
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
J. Davies
中科院分区：
文献类型：
--
作者：
Y. Ujiie;K. Gomi;J. Davies
我们最近报道了使用人脐带血管周围细胞（HUCPVCs），在胶原海绵中交付，在愈合的颅骨截骨免疫抑制大鼠，在体外成骨分化。我们将这些细胞与人骨髓（BM）永生化细胞系进行了比较，并使用后者的条件培养基刺激HUCPVCs的成骨分化。我们的研究结果表明，HUCPVCs提供了统计学显着的骨修复相比，对照组，但未能显示成骨分化在体外。相反，BM细胞在体外成骨试验中表现出高水平的碱性磷酸酶表达，但当加载到胶原支架上并植入时，与假手术或仅胶原对照相比，没有产生统计学上不同的骨量。我们的结果表明，所采用的体外测定不能预测体内结果，并且所采用的BM-MSC细胞系或来自此类细胞的CM与体内单独使用HUCPVC相比没有成骨优势。在这里，我们讨论了这些发现的MSC的功能表型的背景下，采用的测定，供体的年龄，和细胞的组织来源的矛盾的结果。从治疗的角度来看，很明显，只有体内测定应被用作临床表现的预测因子，并且来源于源自年轻供体的组织的细胞可能在功能上比成人组织来源的MSC来源更有效。
We recently reported the use of human umbilical cord perivascular cells (HUCPVCs), delivered in a collagen sponge, in the healing of calvarial osteotomies in immune-suppressed rats, following their osteogenic differentiation in vitro. We compared these cells with a human bone marrow (BM) immortalized cell line, and also used conditioned medium from the latter to stimulate the osteogenic differentiation of the HUCPVCs. Our results showed that HUCPVCs provided statistically significant bony repair compared to controls, but failed to show osteogenic differentiation in vitro. On the contrary, BM cells exhibited high levels of alkaline phosphatase expression in vitro, in osteogenic assays, but when loaded onto collagen scaffolds and implanted did not produce a statistically different amount of bone from either sham or collagen-only controls. Our results demonstrated that the in vitro assays employed did not predict in vivo outcomes, and that the BM-MSC cell line employed, or CM from such cells, provided no osteogenic advantage over the use of HUCPVCs alone in vivo. Here, we discuss these findings in light of the contradictory findings of the functional phenotype of MSC within the context of the assay employed, the age of the donor, and the tissue source of the cells. From a therapeutic perspective, it is clear that only in vivo assays should be employed as predictors of clinical performance and that cells sourced from tissue originating from younger donors may be functionally more potent than adult tissue derived MSC sources.
影响因子：
4.1
作者：
Osugi, Masashi;Katagiri, Wataru;Ueda, Minoru
通讯作者：
Ueda, Minoru