Atrial natriuretic peptide and sodium azide dependent guanylate cyclase activities in placentas from normal and severely toxemic patients
Atrial natriuretic peptide and sodium azide dependent guanylate cyclase activities in placentas from normal and severely toxemic patients
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正常和严重毒血症患者胎盘中心房钠尿肽和叠氮化钠依赖性鸟苷酸环化酶活性
DOI：
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发表时间：
1989
影响因子：
2.4
通讯作者：
J. D. Bottoms
中科院分区：
文献类型：
--
作者：
C. Hatjis;J. D. Bottoms
颗粒鸟苷酸环化酶通过受体依赖性和亚硝基血管扩张剂通过受体非依赖性机制受到几种激素的刺激。心房钠素肽（ANP）受体的一个亚型与鸟苷酸环化酶偶联。研究表明，严重毒血症患者胎盘质膜中ANP受体对α - hanp的亲和力下调。我们想知道这些变化是否与anp依赖性鸟苷酸环化酶活性的下调有关。鸟苷酸环化酶是用正常和严重中毒患者的胎盘质膜片段进行体外实验测定的。存在anp依赖性的胎盘鸟苷酸环化酶活性被证明在正常和毒血症胎盘。虽然基础鸟苷酸环化酶活性不受妊娠毒血症的影响，但α - hanp对该酶的最大刺激显著降低（正常和毒血症分别为104.81 +/- 12.02% （n = 4）和49.41 +/- 8.73% (n = 7)）。最后，亚硝基血管扩张剂叠氮化钠（NaN3）对毒血症胎盘的刺激也低于正常对照组。这些观察结果扩展了我们之前报道的胎盘ANP受体功能的结果，但也表明在毒血症胎盘中鸟苷酸环化酶活性可能存在受体独立的降低。
Particulate guanylate cyclase is stimulated by several hormones through receptor-dependent and by nitrosovasodilators through receptor-independent mechanisms. A subtype of atrial natriuretic peptide (ANP) receptors is coupled to guanylate cyclase. It has been shown that there is a down-regulation of the affinity of ANP receptors to alpha-hANP in placental plasma membranes obtained from severely toxemic patients. We have asked the question whether these changes are associated with a down-regulation of ANP-dependent guanylate cyclase activity. Guanylate cyclase was determined by in vitro experiments using a placental plasma membrane fraction obtained from normal and from severely toxemic patients. The presence of ANP-dependent placental guanylate cyclase activity was demonstrated both in normal and toxemic placentas. Although basal guanylate cyclase activity was not influenced by toxemia of pregnancy, there was a significant decrease in the maximum stimulation of this enzyme by alpha-hANP (104.81 +/- 12.02% (n = 4) vs 49.41 +/- 8.73% (n = 7) for normal and toxemics, respectively). Finally, stimulation by a nitrosovasodilator, sodium azide (NaN3), was also lower in toxemic placentas than in normal controls. These observations extend our previously reported results on placental ANP receptor function but also suggest the presence of a possibly receptor-independent decrease in guanylate cyclase activity in toxemic placentas.
心房钠尿因子诱导人胎盘膜蛋白磷酸化：鸟苷 3:5-环单磷酸模仿的效果。
DOI：
10.1016/s0006-291x(86)80009-2
发表时间：
1986
影响因子：
3.1
作者：
Sen,I;Roy,P
通讯作者：
Roy,P
人胎盘中心房钠尿因子受体的鉴定和溶解。
DOI：
10.1016/0006-291x(86)90019-7
发表时间：
1986
影响因子：
3.1
作者：
Sen,I
通讯作者：
Sen,I