A flax fibre proteome: identification of proteins enriched in bast fibres.
A flax fibre proteome: identification of proteins enriched in bast fibres.
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DOI：
10.1186/1471-2229-8-52
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发表时间：
2008-04-30
影响因子：
5.3
通讯作者：
Deyholos MK
中科院分区：
文献类型：
--
作者：
Hotte NS;Deyholos MK
亚麻韧皮部组织中的韧皮纤维具有科学价值和经济价值，部分原因是次生细胞壁沉积的动态系统。为了更好地了解亚麻细胞壁发育过程中潜在的分子机制，我们在次生细胞壁发育的早期阶段从单独解剖的韧皮部纤维(即单个细胞)中提取蛋白质，并使用荧光(DGE)标记和2D-Gel电泳法将这些蛋白质提取物与周围皮质非纤维细胞的蛋白质提取物进行比较，并通过质谱仪对一些蛋白质进行鉴定。纤维中许多蛋白质的丰度与周围皮质非纤维细胞显著不同，在1,850个可检测到的斑点中，约有13%显著(>；1.5倍，p≤0.05)富含纤维。在质谱学之后，我们对114个点进行了鉴定，其中51个点的纤维含量显著丰富。我们观察到，K+通道亚基、膜联蛋白、孔蛋白、分泌途径成分、β-淀粉酶、β-半乳糖苷酶、果胶和半乳糖生物合成酶是在发育中的亚麻纤维中检测到的最高丰度的蛋白质，其中许多蛋白质显示出与翻译后修饰一致的电泳谱。我们鉴定的富含纤维的蛋白质与组织学和生化分析先前提出的次生壁沉积的动态过程是一致的，特别是半乳糖和分泌途径在这一过程中的重要性。β-淀粉酶的明显丰富表明，淀粉可能是亚麻韧皮纤维细胞壁生物发生的一种未被重视的材料来源。此外，我们的观察证实了先前的报道，即积累蛋白如膜联蛋白和特定的热休克蛋白与次生细胞壁沉积有关。
Bast fibres from the phloem tissues of flax are scientifically interesting and economically useful due in part to a dynamic system of secondary cell wall deposition. To better understand the molecular mechanisms underlying the process of cell wall development in flax, we extracted proteins from individually dissected phloem fibres (i.e. individual cells) at an early stage of secondary cell wall development, and compared these extracts to protein extracts from surrounding, non-fibre cells of the cortex, using fluorescent (DiGE) labels and 2D-gel electrophoresis, with identities assigned to some proteins by mass spectrometry. The abundance of many proteins in fibres was notably different from the surrounding non-fibre cells of the cortex, with approximately 13% of the 1,850 detectable spots being significantly (> 1.5 fold, p ≤ 0.05) enriched in fibres. Following mass spectrometry, we assigned identity to 114 spots, of which 51 were significantly enriched in fibres. We observed that a K+ channel subunit, annexins, porins, secretory pathway components, β-amylase, β-galactosidase and pectin and galactan biosynthetic enzymes were among the most highly enriched proteins detected in developing flax fibres, with many of these proteins showing electrophoretic patterns consistent with post-translational modifications. The fibre-enriched proteins we identified are consistent with the dynamic process of secondary wall deposition previously suggested by histological and biochemical analyses, and particularly the importance of galactans and the secretory pathway in this process. The apparent abundance of β-amylase suggests that starch may be an unappreciated source of materials for cell wall biogenesis in flax bast fibres. Furthermore, our observations confirm previous reports that correlate accumulation proteins such as annexins, and specific heat shock proteins with secondary cell wall deposition.
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影响因子：
4.3
作者：
Day, A;Ruel, K;Chabbert, B
通讯作者：
Chabbert, B
DOI：
10.1046/j.1432-1033.2003.03612.x
发表时间：
2003-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Hofmann, A;Delmer, DP;Wlodawer, A
通讯作者：
Wlodawer, A
影响因子：
3.4
作者：
Bayer, EM;Bottrill, AR;Maule, AJ
通讯作者：
Maule, AJ
影响因子：
4.3
作者：
Blee, KA;Wheatley, ER;Bolwell, GP
通讯作者：
Bolwell, GP
影响因子：
4.2
作者：
Gorshkova, TA;Salnikov, VV;Lozovaya, VV
通讯作者：
Lozovaya, VV