Rapid spread of tomato yellow leaf curl virus in China is aided differentially by two invasive whiteflies.
Rapid spread of tomato yellow leaf curl virus in China is aided differentially by two invasive whiteflies.
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两种入侵粉虱对番茄黄化曲叶病毒在中国的快速传播有不同的帮助
DOI：
10.1371/journal.pone.0034817
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Pan H;Chu D;Yan W;Su Q;Liu B;Wang S;Wu Q;Xie W;Jiao X;Li R;Yang N;Yang X;Xu B;Brown JK;Zhou X;Zhang Y
番茄黄化曲叶病毒（TYLCV）于2006年传入中国，比入侵烟粉虱Bemisia tabaci（Genn.）B型。即便如此，TYLCV的分布和流行仍然有限，经济损失也很小。2003年Q型病毒传入中国后，TYLCV的流行和传播开始加速。这导致了这两种生物型可能不是TYLCV的同等能力载体的假设。方法/主要发现TYLCV在田间采集的B中的感染频率。在实验室条件下，对烟粉虱B型和Q型对TYLCV的感染和传播能力进行了比较研究。分析B。在中国18个省、自治区、直辖市的55个烟粉虱种群中，存在12个B型和43个Q型。获得和传播实验表明，B和Q生物型都能获得和传播病毒，但Q生物型的获得和传播能力上级B生物型。具体而言，Q生物型比B生物型获得显著更多的病毒DNA，并且在显著更短的时间内达到最大病毒载量。虽然TYLCV被证明是水平传播的两种生物型，Q生物型表现出显着更高的病毒传播频率比B生物型。垂直传播结果表明，TYLCV DNA在卵和成虫中均能检测到，但在第一代子代的蛹和成虫中均未检测到。结论/意义这些综合结果表明，TYLCV的流行病学是由两个入侵粉虱（B和Q生物型）通过水平，而不是垂直传播的病毒的差异帮助。这与最近Q型粉虱在中国的快速入侵之后，TYLCV在番茄田中的伴随爆发是一致的。
Background Tomato yellow leaf curl virus (TYLCV) was introduced into China in 2006, approximately 10 years after the introduction of an invasive whitefly, Bemisia tabaci (Genn.) B biotype. Even so the distribution and prevalence of TYLCV remained limited, and the economic damage was minimal. Following the introduction of Q biotype into China in 2003, the prevalence and spread of TYLCV started to accelerate. This has lead to the hypothesis that the two biotypes might not be equally competent vectors of TYLCV. Methodology/Principal Findings The infection frequency of TYLCV in the field-collected B. tabaci populations was investigated, the acquisition and transmission capability of TYLCV by B and Q biotypes were compared under the laboratory conditions. Analysis of B. tabaci populations from 55 field sites revealed the existence of 12 B and 43 Q biotypes across 18 provinces in China. The acquisition and transmission experiments showed that both B and Q biotypes can acquire and transmit the virus, however, Q biotype demonstrated superior acquisition and transmission capability than its B counterparts. Specifically, Q biotype acquired significantly more viral DNA than the B biotype, and reached the maximum viral load in a substantially shorter period of time. Although TYLCV was shown to be transmitted horizontally by both biotypes, Q biotype exhibited significantly higher viral transmission frequency than B biotype. Vertical transmission result, on the other hand, indicated that TYLCV DNA can be detected in eggs and nymphs, but not in pupae and adults of the first generation progeny. Conclusions/Significance These combined results suggested that the epidemiology of TYLCV was aided differentially by the two invasive whiteflies (B and Q biotypes) through horizontal but not vertical transmission of the virus. This is consistent with the concomitant eruption of TYLCV in tomato fields following the recent rapid invasion of Q biotype whitefly in China.
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影响因子：
5
作者：
Brown, JK
通讯作者：
Brown, JK
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作者：
Ghanim, M;Czosnek, H
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Czosnek, H
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