A polarized epithelium organized by beta- and alpha-catenin predates cadherin and metazoan origins.-猫眼课题宝

DOI：

10.1126/science.1199633

发表时间：

2011-03-11

期刊：

Science (New York, N.Y.)

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Dickinson DJ;Nelson WJ;Weis WI

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152298/?report=classic

非后生动物盘基网柄藻中的极化上皮需要α-连环蛋白和β-连环蛋白，但不需要经典的钙粘蛋白、极性蛋白或Wnt信号传导。后生动物的一个基本特征是形成一个简单的极化上皮。在高等动物中，简单上皮的结构完整性和功能极化需要含有经典钙粘蛋白、Wnt信号蛋白β-连环蛋白和肌动蛋白结合蛋白α-连环蛋白的细胞-细胞粘附复合物。我们发现，非后生动物盘基网骨藻形成了一个极化的上皮细胞，是必不可少的多细胞发展。虽然D.由于盘状突缺乏钙粘蛋白同源物，我们鉴定了结合β-连环蛋白相关蛋白的α-连环蛋白直系同源物。这两种蛋白质都是上皮形成、极化蛋白分泌和适当的多细胞形态发生所必需的。因此，后生动物多细胞性的组织原则可能比以前认识到的更古老，并且连环蛋白在细胞极性中的作用早于Wnt信号传导和经典钙粘蛋白的进化。

A polarized epithelium in the non-metazoan Dictyostelium discoideum requires α-catenin and β-catenin but not classical cadherins, polarity proteins or Wnt signaling. A fundamental characteristic of metazoans is the formation of a simple, polarized epithelium. In higher animals, the structural integrity and functional polarization of simple epithelia require a cell-cell adhesion complex containing a classical cadherin, the Wnt-signaling protein β-catenin and the actin-binding protein α-catenin. We show that the non-metazoan Dictyostelium discoideum forms a polarized epithelium that is essential for multicellular development. Although D. discoideum lacks a cadherin homolog, we identify an α-catenin ortholog that binds a β-catenin-related protein. Both proteins are essential for formation of the epithelium, polarized protein secretion and proper multicellular morphogenesis. Thus the organizational principles of metazoan multicellularity may be more ancient than previously recognized, and the role of the catenins in cell polarity predates the evolution of Wnt signaling and classical cadherins.