Immune Cell Infiltration Analysis Demonstrates Excessive Mast Cell Activation in Psoriasis.
Immune Cell Infiltration Analysis Demonstrates Excessive Mast Cell Activation in Psoriasis.
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免疫细胞浸润分析表明牛皮癣中肥大细胞过度激活
DOI：
10.3389/fimmu.2021.773280
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发表时间：
2021
影响因子：
7.3
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Zhang Y;Shi Y;Lin J;Li X;Yang B;Zhou J
银屑病体现了多种炎症过程，以及先天和适应性免疫成分针对上皮损伤产生的过度生理反应，因此，对比患病皮肤与健康皮肤中的免疫细胞生态位至关重要。在此，我们利用CIBERSORT算法，以基因表达谱作为输入数据，推断出银屑病皮肤和健康皮肤中不同免疫细胞类型的占比。结果显示，与健康皮肤相比，银屑病皮肤中的免疫细胞谱发生了显著变化。有趣的是，静息态肥大细胞在银屑病皮肤中几乎消失不见。相反，活化态肥大细胞在银屑病皮肤中富集，这表明肥大细胞的活化可能在银屑病发病机制中发挥重要作用。此外，我们发现，经依那西普治疗后，患病皮肤中静息态肥大细胞的占比逐渐恢复至正常水平，这表明肥大细胞在维持免疫细胞生态位方面起着关键作用。进一步实验证实，与健康皮肤相比，银屑病皮肤中的肥大细胞数量显著减少，且肥大细胞过度活化。总之，我们对免疫细胞谱及相应标记基因表达的综合分析，有助于更好地理解银屑病中的炎症反应，并为临床应用提供了重要线索。
Psoriasis represents multiple inflammatory processes and exaggerated physiological responses to epithelial damage by innate and adaptive immune components, thus it is critical to compare the immune cell niche in disease and healthy skin. Here, we inferred the proportions of different immune cell types in psoriatic and healthy skin using the CIBERSORT algorithm with expression profiles as input. As a result, we observed a dramatic change of immune cell profiles in psoriatic skin compared with healthy skin. Interestingly, the resting mast cells is almost eliminated in psoriatic skin. In contrast, the activated mast cells are enriched in psoriatic skin, indicating that mast cells activation may play an important role in psoriasis pathogenesis. In addition, we found that the proportion of the resting mast cells gradually come back to the normal level in lesioned skin upon etanercept treatment, suggesting that mast cells play a critical role in immune cell niche maintenance. Further experiments validated a significant decrease in mast cell population and an excessive mast cell activation in psoriatic skin compared with healthy skin. In conclusion, our integrative analyses of the immune cell profiles and the corresponding marker genes expression provide a better understanding of the inflammation response in psoriasis and important clues for clinical applications.
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影响因子：
16
作者：
Giordani, Lorenzo;He, Gary J.;Le Grand, Fabien
通讯作者：
Le Grand, Fabien
影响因子：
7.7
作者：
Racle J;de Jonge K;Baumgaertner P;Speiser DE;Gfeller D
通讯作者：
Gfeller D
影响因子：
9.3
作者：
Baron M;Veres A;Wolock SL;Faust AL;Gaujoux R;Vetere A;Ryu JH;Wagner BK;Shen-Orr SS;Klein AM;Melton DA;Yanai I
通讯作者：
Yanai I
DOI：
10.4049/jimmunol.1100123
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lin AM;Rubin CJ;Khandpur R;Wang JY;Riblett M;Yalavarthi S;Villanueva EC;Shah P;Kaplan MJ;Bruce AT
通讯作者：
Bruce AT
影响因子：
32.4
作者：
Cildir, Gokhan;Toubia, John;Tergaonkar, Vinay
通讯作者：
Tergaonkar, Vinay