Sepsis induces variation of intestinal barrier function in different phase through nuclear factor kappa B signaling.
Sepsis induces variation of intestinal barrier function in different phase through nuclear factor kappa B signaling.
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DOI：
10.4196/kjpp.2021.25.4.375
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Lu WH
中科院分区：
文献类型：
--
作者：
Cao YY;Wang ZH;Xu QC;Chen Q;Wang Z;Lu WH
脓毒症时肠屏障功能紊乱，而对脓毒症不同阶段的变化知之甚少。本研究采用盲肠结扎穿刺法(CLP)建立小鼠脓毒症模型。观察CLP后不同时间点的切片HE染色和血清二胺氧化酶浓度。TUNEL法和EDU染色检测肠上皮细胞的凋亡和增殖情况。用Western blotting检测相关蛋白表达，用ELISA法测定血清促炎细胞因子浓度。脓毒症后24 h肠屏障破坏加重，随后24 h逐渐恢复，细胞凋亡率前24 h升高，48 h下降，肠上皮细胞增殖率前6 h受抑，后期恢复。核因子-κB活性与肠屏障功能变化趋势相似，与肠上皮细胞凋亡呈正相关。这些发现揭示了脓毒症时肠屏障功能的转换过程，并且这一过程与NF-κB信号的活性密切相关。
The intestinal barrier function disrupted in sepsis, while little is known about the variation in different phases of sepsis. In this study, mouse models of sepsis were established by caecal ligation and puncture (CLP). The H&E staining of sections and serum diamine oxidase concentration were evaluated at different timepoint after CLP. TUNEL assay and EdU staining were performed to evaluate the apoptosis and proliferation of intestinal epithelium. Relative protein expression was assessed by Western blotting and serum concentrations of pro-inflammatory cytokines was measured by ELISA. The disruption of intestinal barrier worsened in the first 24 h after the onset of sepsis and gradually recovered over the next 24 h. The percentage of apoptotic cell increased in the first 24 h and dropped at 48 h, accompanied with the proliferative rate of intestinal epithelium inhibited in the first 6 h and regained in the later period. Furthermore, the activity of nuclear factor kappa B (NF-κB) presented similar trend with the intestinal barrier function, shared positive correction with apoptosis of intestinal epithelium. These findings reveal the conversion process of intestinal barrier function in sepsis and this process is closely correlated with the activity of NF-κB signaling.
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