Lipidomics profiling by high-resolution LC-MS and high-energy collisional dissociation fragmentation: focus on characterization of mitochondrial cardiolipins and monolysocardiolipins.
Lipidomics profiling by high-resolution LC-MS and high-energy collisional dissociation fragmentation: focus on characterization of mitochondrial cardiolipins and monolysocardiolipins.
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DOI：
10.1021/ac102598u
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发表时间：
2011-02-01
影响因子：
7.4
通讯作者：
Kristal, Bruce S.
中科院分区：
文献类型：
--
作者：
Bird, Susan S.;Marur, Vasant R.;Sniatynski, Matthew J.;Greenberg, Heather K.;Kristal, Bruce S.
采用液-质联用(LC-MS)方法对生物样品中的脂类进行分离，并对生物样品中的单脂进行定性和半定量检测。使用高分辨率全扫描质谱仪和高能碰撞解离(HCD)全离子碎裂技术获得数据。对该方法在正负电离模式下的高效分离和检测进行了评估，使用了跨越6个脂类的标准。通过提取离子色谱图评估与线粒体脂质心磷脂(CL)相关的平台线性和稳健性，从全扫描精确质量测量中提取质量耐受窗口小于或等于5ppm。平台化学发光检测下限在柱上为5pmol(0.9ppm)，质量准确度为1.5ppm，保留时间变异系数为0.5%，面积变异系数为13%。这种质量准确性对于通过消除假阳性来鉴定线粒体样本中未知的CL物种至关重要。除了检测和相对定量线粒体中的CL物种外，还通过交替使用不同能量的HCD扫描来表征CL结构，以在分析中对所有离子产生诊断碎片。开发的脂谱方法被应用于一项动物研究中的线粒体样本，该研究与饮食、线粒体功能和疾病之间的联系有关。分析确定了28种独特的CL和2种单溶心磷脂，它们经常与线粒体应激和功能障碍有关。
A liquid chromatography-mass spectrometry (LC-MS) method was used for separation of lipid classes as well as both qualitative and semi-quantitative detection of individual lipids in biological samples. Data were acquired using high resolution full scan MS and high energy collisional dissociation (HCD) all ion fragmentation. The method was evaluated for efficient separation and detection in both positive and negative ionization mode using standards spanning 6 lipid classes. Platform linearity and robustness, related to the mitochondrial lipid cardiolipin (CL), were assessed using extracted ion chromatograms with mass tolerance windows of 5ppm or less from full scan exact mass measurements. The platform CL limit of detection was determined to be 5 pmol (0.9 μM) on the column, with mass accuracy <1.5 ppm, retention time coefficients of variation (CV) < 0.5%, and area CV < 13%. This mass accuracy was critical to the identification of unknown CL species in mitochondria samples, through the elimination of false positives. In addition to detection and relative quantitation of CL species in mitochondria, CL structures were characterized through the use of alternating HCD scans at different energies to produce diagnostic fragmentations on all ions in the analysis. The developed lipid profiling method was applied to mitochondrial samples from an animal study related to the linkages between diet, mitochondrial function and disease. The analysis identified 28 unique CL species and 2 monolysocardiolipin species that are often associated with mitochondrial stress and dysfunction.
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影响因子：
3.6
作者：
BARZANTI, V;BATTINO, M;LENAZ, G
通讯作者：
LENAZ, G
影响因子：
2.9
作者：
Han, XL;Yang, JY;Gross, RW
通讯作者：
Gross, RW
影响因子：
2
作者：
Plumb, Robert S.;Johnson, Kelly A.;Nicholson, Jeremy K.
通讯作者：
Nicholson, Jeremy K.
DOI：
10.1073/pnas.0811224106
发表时间：
2009-02-17
影响因子：
11.1
作者：
Malhotra, Ashim;Edelman-Novemsky, Irit;Ren, Mindong
通讯作者：
Ren, Mindong
影响因子：
4.4
作者：
Hu, Chunxiu;van Dommelen, Judith;Hankemeier, Thomas
通讯作者：
Hankemeier, Thomas