Wild type and mutant 2009 pandemic influenza A (H1N1) viruses cause more severe disease and higher mortality in pregnant BALB/c mice.
Wild type and mutant 2009 pandemic influenza A (H1N1) viruses cause more severe disease and higher mortality in pregnant BALB/c mice.
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DOI：
10.1371/journal.pone.0013757
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发表时间：
2010-10-29
期刊：
影响因子：
3.7
通讯作者：
Yuen KY
中科院分区：
文献类型：
--
作者：
Chan KH;Zhang AJ;To KK;Chan CC;Poon VK;Guo K;Ng F;Zhang QW;Leung VH;Cheung AN;Lau CC;Woo PC;Tse H;Wu W;Chen H;Zheng BJ;Yuen KY
孕妇感染2009年甲型H1N1流感病毒后病情更重，死亡率更高，但其发病机制尚不清楚。我们发现，较高的死亡率，更严重的肺炎，更高的肺病毒载量，较低的外周血T淋巴细胞和抗体反应，更高水平的促炎细胞因子和趋化因子，以及更差的胎儿发育发生在怀孕的小鼠比非怀孕的对照组感染的野生型（临床分离株）或小鼠适应的突变病毒与D222 G取代的血凝素。这些疾病相关的变化和下呼吸道受累在突变病毒攻击的妊娠小鼠中更严重。虽然人胎盘来源的JEG-3细胞系可以被感染，并且一些小鼠羊水中的促炎细胞因子或趋化因子升高，但通过培养、实时逆转录聚合酶链反应或组织病理学改变，未检测到病毒累及胎盘或胎儿。病毒核蛋白和Ⅱ型肺泡细胞标志物SP-C蛋白的双重免疫荧光染色表明，大多数感染的肺泡上皮细胞是Ⅱ型肺泡细胞。这种大流行性病毒对母亲和胎儿结局的不利影响在很大程度上与严重的肺部疾病和炎性细胞因子溢出到体循环中的间接影响有关。
Pregnant women infected by the pandemic influenza A (H1N1) 2009 virus had more severe disease and higher mortality but its pathogenesis is still unclear. We showed that higher mortality, more severe pneumonitis, higher pulmonary viral load, lower peripheral blood T lymphocytes and antibody responses, higher levels of proinflammatory cytokines and chemokines, and worse fetal development occurred in pregnant mice than non-pregnant controls infected by either wild type (clinical isolate) or mouse-adapted mutant virus with D222G substitution in hemagglutinin. These disease-associated changes and the lower respiratory tract involvement were worse in pregnant mice challenged by mutant virus. Though human placental origin JEG-3 cell line could be infected and proinflammatory cytokines or chemokines were elevated in amniotic fluid of some mice, no placental or fetal involvement by virus were detected by culture, real-time reverse transcription polymerase chain reaction or histopathological changes. Dual immunofluorescent staining of viral nucleoprotein and type II alveolar cell marker SP-C protein suggested that the majority of infected alveolar epithelial cells were type II pneumocytes. The adverse effect of this pandemic virus on maternal and fetal outcome is largely related to the severe pulmonary disease and the indirect effect of inflammatory cytokine spillover into the systemic circulation.
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影响因子：
168.9
作者：
Jamieson, Denise J.;Honein, Margaret A.;Olsen, Sonja J.
通讯作者：
Olsen, Sonja J.
影响因子：
8.8
作者：
Li, I. W. S.;Chan, K. H.;Yuen, K. Y.
通讯作者：
Yuen, K. Y.
DOI：
10.1136/bmj.c1279
发表时间：
2010-03-18
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
ANZIC Influenza Investigators and Australasian Maternity Outcomes Surveillance System
通讯作者：
ANZIC Influenza Investigators and Australasian Maternity Outcomes Surveillance System
影响因子：
11.8
作者：
To, Kelvin K. W.;Hung, Ivan F. N.;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
158.5
作者：
Louie, Janice K.;Acosta, Meileen;Honein, Margaret A.
通讯作者：
Honein, Margaret A.