Subfunctionalization of sigma factors during the evolution of land plants based on mutant analysis of liverwort (Marchantia polymorpha L.) MpSIG1.
Subfunctionalization of sigma factors during the evolution of land plants based on mutant analysis of liverwort (Marchantia polymorpha L.) MpSIG1.
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DOI：
10.1093/gbe/evt137
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发表时间：
2013
影响因子：
3.3
通讯作者：
Nishimura Y
中科院分区：
文献类型：
--
作者：
Ueda M;Takami T;Peng L;Ishizaki K;Kohchi T;Shikanai T;Nishimura Y
Sigma因子是质体编码的RNA聚合酶的亚基，其通过识别一组启动子来调节质体编码的基因的转录。Sigma因子在陆地植物进化过程中拷贝数增加并多样化，但这一过程的细节仍然未知。苔类是胚状体的基础类群，被认为保留了陆生植物的祖先特征。在地钱（地钱）中，我们分离并鉴定了σ因子1（MpSIG 1）的T-DNA标记的突变体（Mpsig 1）。突变体没有显示出任何可见的表型，这意味着MpSIG 1功能与其他sigma因子的功能是冗余的。然而，定量逆转录聚合酶链反应和RNA凝胶印迹分析显示，与光合作用相关的基因下调，导致一些蛋白质复合物的轻微减少。在petL，psaA，psbB，psbK，和psbE操纵子的苔类植物中聚集的基因的转录水平低于野生型，在水稻（Oryza sativa）中的SIG 1缺陷型突变体的结果相似。过表达分析显示，原始的SIG 1和SIG 2蛋白质之间的功能分歧，而这些蛋白质仍然保留功能冗余。我们还发现，ndhF mRNA表达的主要σ因子在苔类植物、拟南芥和水稻中是多样化的。我们的研究显示了SIG 1的祖先功能和陆地植物进化过程中sigma因子的功能划分（亚功能化）过程。
Sigma factor is a subunit of plastid-encoded RNA polymerase that regulates the transcription of plastid-encoded genes by recognizing a set of promoters. Sigma factors have increased in copy number and have diversified during the evolution of land plants, but details of this process remain unknown. Liverworts represent the basal group of embryophytes and are expected to retain the ancestral features of land plants. In liverwort (Marchantia polymorpha L.), we isolated and characterized a T-DNA-tagged mutant (Mpsig1) of sigma factor 1 (MpSIG1). The mutant did not show any visible phenotypes, implying that MpSIG1 function is redundant with that of other sigma factors. However, quantitative reverse-transcription polymerase chain reaction and RNA gel blot analysis revealed that genes related to photosynthesis were downregulated, resulting in the minor reduction of some protein complexes. The transcript levels of genes clustered in the petL, psaA, psbB, psbK, and psbE operons of liverwort were lower than those in the wild type, a result similar to that in the SIG1 defective mutant in rice (Oryza sativa). Overexpression analysis revealed primitive functional divergence between the SIG1 and SIG2 proteins in bryophytes, whereas these proteins still retain functional redundancy. We also discovered that the predominant sigma factor for ndhF mRNA expression has been diversified in liverwort, Arabidopsis (Arabidopsis thaliana), and rice. Our study shows the ancestral function of SIG1 and the process of functional partitioning (subfunctionalization) of sigma factors during the evolution of land plants.
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影响因子：
5.1
作者：
Lerbs-Mache, Silva
通讯作者：
Lerbs-Mache, Silva
影响因子：
4.9
作者：
Kubota, Yoshiki;Miyao, Akio;Asayama, Munehiko
通讯作者：
Asayama, Munehiko
影响因子：
4.8
作者：
Hakimi, MA;Privat, I;Lerbs-Mache, S
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Lerbs-Mache, S
DOI：
10.1073/pnas.94.26.14948
发表时间：
1997-12-23
影响因子：
11.1
作者：
Isono, K;Shimizu, M;Kobayashi, H
通讯作者：
Kobayashi, H
影响因子：
11.6
作者：
Lai, Zhibing;Li, Ying;Chen, Zhixiang
通讯作者：
Chen, Zhixiang