Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices.
Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices.
复制标题
DOI：
10.1016/j.biomaterials.2021.121350
复制
发表时间：
2022-03
期刊：
影响因子：
14
通讯作者：
Wildman RD
中科院分区：
文献类型：
--
作者：
He Y;Luckett J;Begines B;Dubern JF;Hook AL;Prina E;Rose FRAJ;Tuck CJ;Hague RJM;Irvine DJ;Williams P;Alexander MR;Wildman RD
由于植入的医疗设备上形成细菌生物膜而导致的慢性感染是一个主要的全球医疗问题，需要新的生物兼容、生物膜耐受材料。在这里，我们展示了如何通过基于喷墨的3D打印，使用细菌生物膜抑制配方来制造定制设备，而不需要洗脱抗生素或涂层。配制了候选单体，并证明了它们的加工性和可靠性。根据3D打印结构的体外细菌生物膜抑制特性和缺乏哺乳动物细胞细胞毒性，选择了用于体内评估的配方。在小鼠种植体感染模型中，与医用级硅胶相比，聚TCDMDA上铜绿假单胞菌生物被膜的形成减少了~99%。全鼠生物发光成像和组织免疫组织化学显示，打印设备能够调节宿主免疫反应，并防止设备上生物膜的形成和周围组织的感染。由于3D打印可用于制造原型和临床使用的设备，基于喷墨的3D打印用于创建个性化功能医疗设备的多功能性通过以聚TCDMDA打印的手指关节假体和前列腺癌支架对铜绿假单胞菌和金黄色葡萄球菌的生物膜抵抗力得到证明。
Chronic infection as a result of bacterial biofilm formation on implanted medical devices is a major global healthcare problem requiring new biocompatible, biofilm-resistant materials. Here we demonstrate how bespoke devices can be manufactured through ink-jet-based 3D printing using bacterial biofilm inhibiting formulations without the need for eluting antibiotics or coatings. Candidate monomers were formulated and their processability and reliability demonstrated. Formulations for in vivo evaluation of the 3D printed structures were selected on the basis of their in vitro bacterial biofilm inhibitory properties and lack of mammalian cell cytotoxicity. In vivo in a mouse implant infection model, Pseudomonas aeruginosa biofilm formation on poly-TCDMDA was reduced by ~99% when compared with medical grade silicone. Whole mouse bioluminescence imaging and tissue immunohistochemistry revealed the ability of the printed device to modulate host immune responses as well as preventing biofilm formation on the device and infection of the surrounding tissues. Since 3D printing can be used to manufacture devices for both prototyping and clinical use, the versatility of ink-jet based 3D-printing to create personalised functional medical devices is demonstrated by the biofilm resistance of both a finger joint prosthetic and a prostatic stent printed in poly-TCDMDA towards P. aeruginosa and Staphylococcus aureus.
登录
查看更多内容
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
46.9
作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
通讯作者：
Atala, Anthony
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
影响因子：
2.4
作者：
Bayraktar, HH;Keaveny, TM
通讯作者：
Keaveny, TM
影响因子：
64.8
作者：
Green JJ;Elisseeff JH
通讯作者：
Elisseeff JH