Characterization of human PDGFR-β-positive pericytes from IPF and non-IPF lungs.
Characterization of human PDGFR-β-positive pericytes from IPF and non-IPF lungs.
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来自 IPF 和非 IPF 肺的人类 PDGFR-β 阳性周细胞的特征。
DOI：
10.1152/ajplung.00289.2018
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Schnapp,LynnM
中科院分区：
文献类型：
--
作者：
Wilson,CaroleL;Stephenson,SarahE;Higuero,JeanPaul;Feghali-Bostwick,Carol;Hung,ChiF;Schnapp,LynnM
周细胞通过与内皮细胞的密切相互作用是微血管系统的关键调节细胞。然而，周细胞在组织动态平衡和修复中发挥着额外的作用，部分是通过转化为肌成纤维细胞。肌成纤维细胞积聚是特发性肺纤维化(IPF)等纤维化疾病的标志。为了了解周细胞在人肺纤维化中的作用和贡献，我们基于周细胞的共同标志物--血小板衍生生长因子受体β(PDGFR-β)的表达，从非肺纤维化对照组和肺纤维化肺组织中分离出这些细胞。当在特殊的生长介质中培养时，PDGFR-β+细胞保持了周细胞典型的形态和标志物特征。我们发现IPF周细胞比对照周细胞迁移更快，更容易侵入基底膜基质。细胞暴露于转化生长因子-β，一种主要的纤维化诱导细胞因子，增加了对照组和肺间质纤维化周细胞中α-平滑肌肌动蛋白和细胞外基质基因的表达。鉴于周细胞在体内对系统和组织来源的危险信号做出反应的独特位置，我们用具有病原体相关分子模式(PAMPs)或损伤相关分子模式(DAMP)的激动剂刺激人肺周细胞。对照和IPF肺周细胞都增加了促炎症趋化因子的表达，以响应特定的PAMP和从坏死细胞释放的湿气。我们的结果表明，对照组和IPF肺周细胞对组织损伤以及微生物和纤维化刺激都有反应。然而，IPF周细胞为迁移和基质侵袭做好了准备，这一特征可能有助于这些细胞在肺纤维化中的功能。
Pericytes are key regulators of the microvasculature through their close interactions with the endothelium. However, pericytes play additional roles in tissue homeostasis and repair, in part by transitioning into myofibroblasts. Accumulation of myofibroblasts is a hallmark of fibrotic diseases such as idiopathic pulmonary fibrosis (IPF). To understand the contribution and role of pericytes in human lung fibrosis, we isolated these cells from non-IPF control and IPF lung tissues based on expression of platelet-derived growth factor receptor-β (PDGFR-β), a common marker of pericytes. When cultured in a specialized growth medium, PDGFR-β+ cells retain the morphology and marker profile typical of pericytes. We found that IPF pericytes migrated more rapidly and invaded a basement membrane matrix more readily than control pericytes. Exposure of cells to transforming growth factor-β, a major fibrosis-inducing cytokine, increased expression of α-smooth muscle actin and extracellular matrix genes in both control and IPF pericytes. Given that pericytes are uniquely positioned in vivo to respond to danger signals of both systemic and tissue origin, we stimulated human lung pericytes with agonists having pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Both control and IPF lung pericytes increased expression of proinflammatory chemokines in response to specific PAMPs and DAMPs released from necrotic cells. Our results suggest that control and IPF lung pericytes are poised to react to tissue damage, as well as microbial and fibrotic stimuli. However, IPF pericytes are primed for migration and matrix invasion, features that may contribute to the function of these cells in lung fibrosis.
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影响因子：
23.9
作者：
Guimarães-Camboa N;Cattaneo P;Sun Y;Moore-Morris T;Gu Y;Dalton ND;Rockenstein E;Masliah E;Peterson KL;Stallcup WB;Chen J;Evans SM
通讯作者：
Evans SM
模拟生理和病理条件以研究大脑功能和功能障碍的周细胞生物学
影响因子：
2.4
作者：
Justin Rustenhoven;Leon C. D. Smyth;D. Jansson;P. Schweder;M. Aalderink;Emma L. Scotter;E. Mee;R. Faull;T. Park;M. Dragunow
通讯作者：
M. Dragunow
DOI：
10.1016/j.ajpath.2014.09.013
发表时间：
2015
期刊：
The American journal of pathology
影响因子：
--
作者：
K. Yuan;M. Orcholski;Cristina Panaroni;Eric M. Shuffle;N. Huang;Xinguo Jiang;W. Tian;Eszter K Vladar;Lingli Wang;M. Nicolls;Joy Y Wu;V. A. de Jesus Perez
通讯作者：
K. Yuan;M. Orcholski;Cristina Panaroni;Eric M. Shuffle;N. Huang;Xinguo Jiang;W. Tian;Eszter K Vladar;Lingli Wang;M. Nicolls;Joy Y Wu;V. A. de Jesus Perez
影响因子：
2.9
作者：
Pieper, Christian;Marek, Jasmin Jacqueline;Galla, Hans-Joachim
通讯作者：
Galla, Hans-Joachim
影响因子：
4.1
作者：
Bichsel, Colette A.;Hall, Sean R. R.;Geiser, Thomas
通讯作者：
Geiser, Thomas