Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation.
Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation.
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DOI：
10.3390/jfb14070346
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发表时间：
2023-06-29
影响因子：
4.8
通讯作者：
中科院分区：
文献类型：
--
作者：
纳米凝胶是生物医学应用的候选者，并且核-壳纳米凝胶提供了调节具有广泛降解能力的热响应行为的潜力。这些性能是通过聚（N-异丙基甲基丙烯酰胺）核和聚（N-异丙基丙烯酰胺）壳的组合来实现的，两者都与可降解交联剂N，N′-双（丙烯酰基）胱胺交联。在这项工作中，这些纳米凝胶的降解行为的特点是使用不对称流场流分馏耦合多角度和动态光散射。通过实时监测纳米凝胶的降解产物，可以确定降解的三个不同阶段：纳米凝胶溶胀、纳米凝胶碎裂和纳米凝胶碎片降解。结果表明，核-壳纳米凝胶比它们的非核-壳对应物降解更慢，可能是由于壳中发生更高程度的自交联反应。大多数降解产物的分子量低于10 kDa，这表明它们可能通过肾脏清除。这项研究为生物医学应用中可降解纳米凝胶的设计和表征提供了重要的见解，强调了需要准确的表征技术来测量纳米凝胶降解产物的潜在生物影响。
Nanogels are candidates for biomedical applications, and core-shell nanogels offer the potential to tune thermoresponsive behaviour with the capacity for extensive degradation. These properties were achieved by the combination of a core of poly(N-isopropylmethacrylamide) and a shell of poly(N-isopropylacrylamide), both crosslinked with the degradable crosslinker N,N′-bis(acryloyl)cystamine. In this work, the degradation behaviour of these nanogels was characterised using asymmetric flow field flow fractionation coupled with multi-angle and dynamic light scattering. By monitoring the degradation products of the nanogels in real-time, it was possible to identify three distinct stages of degradation: nanogel swelling, nanogel fragmentation, and nanogel fragment degradation. The results indicate that the core-shell nanogels degrade slower than their non-core-shell counterparts, possibly due to a higher degree of self-crosslinking reactions occurring in the shell. The majority of the degradation products had molecule weights below 10 kDa, which suggests that they may be cleared through the kidneys. This study provides important insights into the design and characterisation of degradable nanogels for biomedical applications, highlighting the need for accurate characterisation techniques to measure the potential biological impact of nanogel degradation products.
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影响因子：
5.6
作者：
Skupin-Mrugalska P;Elvang PA;Brandl M
通讯作者：
Brandl M
通过控制添加可降解交联剂来调节热敏微凝胶的形态、网络结构和降解
影响因子：
2.4
作者：
Cui, Ruiguang;Zhang, Zhijun;Du, Binyang
通讯作者：
Du, Binyang
影响因子：
2.3
作者：
Garner, Brett W.;Cai, Tong;Neogi, Arup
通讯作者：
Neogi, Arup
影响因子：
2.4
作者：
Gaulding, Jeffrey C.;South, Antoinette B.;Lyon, L. Andrew
通讯作者：
Lyon, L. Andrew
DOI：
10.1073/pnas.1600816113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Nuhn, Lutz;Vanparijs, Nane;De Geest, Bruno G.
通讯作者：
De Geest, Bruno G.