An inducible model of abacterial prostatitis induces antigen specific inflammatory and proliferative changes in the murine prostate.
An inducible model of abacterial prostatitis induces antigen specific inflammatory and proliferative changes in the murine prostate.
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DOI：
10.1002/pros.21327
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发表时间：
2011-08-01
期刊：
影响因子：
2.8
通讯作者：
Ratliff, Timothy L.
中科院分区：
文献类型：
--
作者：
Haverkamp, Jessica M.;Charbonneau, Bridget;Crist, Scott A.;Meyerholz, David K.;Cohen, Michael B.;Snyder, Paul W.;Svensson, Robert U.;Henry, Michael D.;Wang, Hsing-Hui;Ratliff, Timothy L.
关键词：
前列腺炎是一种人们知之甚少的疾病，越来越多的证据表明炎症与其他前列腺疾病有关，包括前列腺癌。通过过继转移至 POET-3 小鼠或 POET-3/Luc/Pten-/+ 小鼠中来检查预激活的 CD8 T 细胞诱导前列腺炎的能力。通过组织学分析、流式细胞术检查白细胞浸润以及评估前列腺组织中的细胞因子和趋化因子水平来确定炎症反应的特征。通过监测上皮细胞随时间的增殖情况来评估炎症对前列腺的影响。卵清蛋白特异性 CD8+ T 细胞（OT-I 细胞）引发的炎症导致 POET-3 和 POET-3/Luc/Pten−/+ 小鼠的前前列腺、背外侧和前前列腺发生急性前列腺炎。急性前列腺炎的特点是募集过继转移的 OT-I 细胞，重要的是，募集自体 CD4+ 和 CD8+ T 细胞、骨髓源性抑制细胞 (MDSC) 和调节性 T 细胞 (Treg)。与白细胞浸润相一致，观察到促炎细胞因子和趋化因子水平升高。炎症还导致显着的上皮细胞增殖，这种增殖在 OT-I 细胞过继转移后可持续长达 80 天。 POET-3 模型代表了一种新颖的小鼠模型，用于在抗原特异性系统中研究急性和慢性前列腺炎症。此外，POET-3小鼠模型可以与其他疾病遗传模型（例如前列腺癌的C57/Luc/Pten−/−模型）交叉，从而可以评估前列腺炎对其他前列腺疾病的影响。
Prostatitis is a poorly understood disease and increasing evidence suggests inflammation is involved in other prostatic diseases, including prostate cancer. The ability of pre-activated CD8 T cells to induce prostatitis was examined by adoptive transfer into POET-3 mice or POET-3/Luc/Pten−/+ mice. Characterization of the inflammatory response was determined by examining leukocyte infiltration by histological analysis, flow cytometry and by evaluating cytokine and chemokine levels in prostate tissue. The impact of inflammation on the prostate was evaluated by monitoring epithelial cell proliferation over time. Initiation of inflammation by ovalbumin specific CD8+ T cells (OT-I cells) resulted in development of acute prostatitis in the anterior, dorsolateral and anterior prostate of POET-3 and POET-3/Luc/Pten−/+ mice. Acute prostatitis was characterized by recruitment of adoptively transferred OT-I cells and importantly, autologous CD4+ and CD8+ T cells, myeloid-derived suppressor cells (MDSC) and regulatory T cells (Treg). In concert with leukocyte infiltration elevated levels of pro-inflammatory cytokines and chemokines were observed. Inflammation also resulted in marked epithelial cell proliferation that was sustained up to 80 days post adoptive-transfer of OT-I cells. The POET-3 model represents a novel mouse model to study both acute and chronic prostate inflammation in an antigen-specific system. Further, the POET-3 mouse model can be crossed with other genetic models of disease such as the C57/Luc/Pten−/− model of prostate cancer, allowing the impact of prostatitis on other prostatic diseases to be evaluated.
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影响因子：
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