Choline‐mediated modulation of hippocampal sharp wave–ripple complexes in vitro
Choline‐mediated modulation of hippocampal sharp wave–ripple complexes in vitro
复制标题
体外胆碱介导的海马尖波波纹复合物的调节
DOI：
10.1111/jnc.12693
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发表时间：
2014
影响因子：
4.7
通讯作者：
Egorov AV
中科院分区：
文献类型：
--
作者：
Fischer V;Both M;Draguhn A;Egorov AV
胆碱能系统关键地参与认知功能的调节，包括学习和记忆。乙酰胆碱通过毒蕈碱受体（mAChR）和烟碱受体（nAChR）起作用，这两种受体都在海马体中大量表达。以前的证据表明，胆碱，乙酰胆碱的前体和降解产物，本身可以激活nAChR，从而影响内在和突触神经元功能。在这里，我们询问胆碱的细胞作用是否直接影响海马网络活动。使用小鼠海马切片，我们发现胆碱有效地抑制自发发生的尖波波纹复合物（SPW-R），并可诱导γ振荡。此外，胆碱减少海马子区域CA 3和CA 1之间的突触传递。令人惊讶的是，这些作用是由mAChR和含α7的nAChR的激活介导的。大多数烟碱效应仅在局部快速应用胆碱后才变得明显，表明nAChRs的快速脱敏动力学。阻断胆碱摄取后仍存在影响，因此，可能是由于胆碱对相应受体的直接作用。总之，胆碱被证明是海马体内模式化网络活动的有效调节剂。这些行动可能是重要的理解在正常和病理改变neuronalnetworks.In这项研究中，我们问胆碱，乙酰胆碱的前体和降解产物，直接影响海马网络的活动状态转换。使用小鼠海马切片，我们发现胆碱有效地抑制自发发生的尖波波纹复合物（SPW-R）。此外，胆碱减少海马子场之间的突触传递。这些作用是通过直接激活毒蕈碱和烟碱胆碱能途径介导的。总之，胆碱被证明是海马网络内模式活动的有效调节剂。
The cholinergic system is critically involved in the modulation of cognitive functions, including learning and memory. Acetylcholine acts through muscarinic (mAChRs) and nicotinic receptors (nAChRs), which are both abundantly expressed in the hippocampus. Previous evidence indicates that choline, the precursor and degradation product of Acetylcholine, can itself activate nAChRs and thereby affects intrinsic and synaptic neuronal functions. Here, we asked whether the cellular actions of choline directly affect hippocampal network activity. Using mouse hippocampal slices we found that choline efficiently suppresses spontaneously occurring sharp wave–ripple complexes (SPW‐R) and can induce gamma oscillations. In addition, choline reduces synaptic transmission between hippocampal subfields CA3 and CA1. Surprisingly, these effects are mediated by activation of both mAChRs and α7‐containing nAChRs. Most nicotinic effects became only apparent after local, fast application of choline, indicating rapid desensitization kinetics of nAChRs. Effects were still present following block of choline uptake and are, therefore, likely because of direct actions of choline at the respective receptors. Together, choline turns out to be a potent regulator of patterned network activity within the hippocampus. These actions may be of importance for understanding state transitions in normal and pathologically altered neuronal networks.In this study we asked whether choline, the precursor and degradation product of acetylcholine, directly affects hippocampal network activity. Using mouse hippocampal slices we found that choline efficiently suppresses spontaneously occurring sharp wave–ripple complexes (SPW‐R). In addition, choline reduces synaptic transmission between hippocampal subfields. These effects are mediated by direct activation of muscarinic as well as nicotinic cholinergic pathways. Together, choline turns out to be a potent regulator of patterned activity within hippocampal networks.
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影响因子：
2.5
作者：
M. Alkondon;E. Albuquerque
通讯作者：
M. Alkondon;E. Albuquerque
影响因子：
3.6
作者：
J. Mielke;T. Ahuja;T. Comas;G. Mealing
通讯作者：
G. Mealing
影响因子：
4.8
作者：
Mellott, TJ;Williams, CL;Blusztajn, JK
通讯作者：
Blusztajn, JK
影响因子：
5.7
作者：
O'Keefe, J
通讯作者：
O'Keefe, J
毒蕈碱激活可减少大鼠海马切片突触传递受阻期间刺激 Schaffer 侧支引起的 [Ca2+]0 变化
影响因子：
2.5
作者：
A. Egorov;U. Heinemann;W. Müller
通讯作者：
W. Müller