Sialic acid recognition is a key determinant of influenza A virus tropism in murine trachea epithelial cell cultures.
Sialic acid recognition is a key determinant of influenza A virus tropism in murine trachea epithelial cell cultures.
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DOI：
10.1016/j.virol.2009.01.005
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发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
Lutz, Andrew
中科院分区：
文献类型：
--
作者：
Pekosz, Andrew;Newby, Celeste;Bose, Pulkit S.;Lutz, Andrew
Influenza A virus interacts with specific types of sialic acid during attachment and entry into susceptible cells. The precise amino acids in the hemagglutinin protein that control sialic acid binding specificity and affinity vary among antigenic subtypes. For H3 subtypes, amino acids 226 and 228 are critical for differentiating between α2,3- and α2,6-linked forms of sialic acid (SA). We demonstrate that position 190 of the HA from A/Udorn/307/72 (H3N2) plays an important role in the recognition of α2,3-SA, as changing the residue from a glutamic acid to an aspartic acid led to alteration of red blood cell hemagglutination and a complete loss of replication in differentiated, murine trachea epithelial cell cultures which express only α2,3-SA. This amino acid change had a minimal effect on virus replication in MDCK cells, suggesting subtle changes in receptor recognition by the H3 hemagglutinin can lead to significant alterations in cell and species tropism.
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影响因子：
3.7
作者：
Newby, Celeste M.;Rowe, Regina K.;Pekosz, Andrew
通讯作者：
Pekosz, Andrew
影响因子：
3.7
作者：
Govorkova, EA;Matrosovich, MN;Webster, RG
通讯作者：
Webster, RG
DOI：
10.1073/pnas.0405172102
发表时间：
2004-12-28
影响因子：
11.1
作者：
Chu, VC;Whittaker, GR
通讯作者：
Whittaker, GR
影响因子：
4.8
作者：
Kumari, Kshama;Gulati, Shelly;Air, Gillian M.
通讯作者：
Air, Gillian M.
影响因子：
2.7
作者：
Medeiros, R;Naffakh, N;van der Werf, S
通讯作者：
van der Werf, S