Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography.
Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography.
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DOI：
10.1016/j.biomaterials.2010.06.042
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Jacques SL
中科院分区：
文献类型：
--
作者：
Levitz D;Hinds MT;Ardeshiri A;Hanson SR;Jacques SL
基质重塑在生理和病理过程以及组织工程应用中起着基础作用。本文应用光学相干断层扫描技术（OCT）对平滑肌细胞（SMCs）胶原凝胶重塑过程进行了成像研究。胶原-SMC凝胶的光学散射特性的定量特征在于通过拟合OCT数据的理论模型。超过5天的基质重塑产生了10倍的胶原蛋白凝胶的反射率增加，对应于从0.91到0.46的散射各向异性降低。反射率的增加在共焦镶嵌图像中得到证实。阻断胶原-SMC凝胶中的基质降解与强力霉素，一种非特异性基质金属蛋白酶（MMPs）抑制剂，阻碍了散射各向异性的降低，导致重塑的宏观迹象很少。用MMP-8（胶原酶2）处理3小时在无细胞凝胶中引起基质降解，部分模拟了5天后在胶原-SMC凝胶中测得的各向异性降低。这些结果表明，胶原-SMC凝胶中散射各向异性的降低是由于MMP活性将胶原原纤维降解成更小的片段。
Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a nondestructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen-SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen-SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 hr treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen-SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen-SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.
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DOI：
10.1083/jcb.149.6.1309
发表时间：
2000-06-12
期刊：
The Journal of cell biology
影响因子：
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作者：
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