The 70-kDa Heat Shock Proteins Associate with Glandular Intermediate Filaments in an ATP-dependent Manner (*)
The 70-kDa Heat Shock Proteins Associate with Glandular Intermediate Filaments in an ATP-dependent Manner (*)
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70 kDa 热休克蛋白以 ATP 依赖性方式与腺中间丝相关 (*)
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
M. Omary
中科院分区：
文献类型：
--
作者：
J. Liao;L. A. Lowthert;N. Ghori;M. Omary
角蛋白多肽8和18 （K8/18）是腺上皮中优先表达的中间丝蛋白。我们描述了通过从正常人类结肠组织和培养细胞系中共免疫沉淀鉴定70 kda热休克蛋白（hsp）及其相关热休克同源蛋白为k8 /18相关蛋白（hsp/c）。这种关联是显著的，但是亚化学计量的，并且优先发生在可溶性组分而不是细胞骨架K8/18组分。热应激增加可溶性K8/18水平，同时增加hsp70水平和K8/18-hsp70关联的化学计量量。通过对纯化的相关蛋白进行胰蛋白酶消化的微测序和使用抗hsp/c70特异性抗体，证实了相关蛋白的身份。K8/18-hsp/c70配合物可以以mg -ATP依赖的方式解离，需要ATP水解。用纯化的牛hsp70和人K8/18免疫沉淀可以重建hsp与K8/18的结合，这些免疫沉淀已经耗尽了结合的hsp/c70，并且在ATP的存在下略有增加。重组的K8/18-hsp70配合物在Mg-ATP存在下可再次释放。此外，在K8/18组装过程中或组装后添加hsp70对体外丝组装没有显著影响。使用覆盖试验，hsp70在ATP存在下只与K8结合。我们的结果表明hsp/c70蛋白与K8/18直接相关。这种相互作用至少在一定程度上可以调节这两个丰富的蛋白质群的功能。
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament proteins expressed preferentially in glandular epithelia. We describe the identification, by co-immunoprecipitation from normal human colonic tissues and cultured cell lines, of the 70-kDa heat shock protein (hsp) and its related heat shock cognate protein as K8/18-associated proteins (hsp/c). The association is significant but sub-stoichiometric and occurs preferentially with the soluble rather than the cytoskeletal K8/18 fractions. Heat stress increases the level of soluble K8/18 in association with an increase in hsp70 levels and an increase in the stoichiometry of K8/18-hsp70 association. Identity of the associated proteins was confirmed by microsequencing of a tryptic digest of the purified associated protein and by using anti-hsp/c70-specific antibodies. The K8/18-hsp/c70 complex can be dissociated in a Mg-ATP-dependent manner that requires ATP hydrolysis. Binding of hsp to K8/18 can be reconstituted using purified bovine hsp70 and human K8/18 immunoprecipitates that have been depleted of bound hsp/c70 and increases slightly in the presence of ATP. The reconstituted K8/18-hsp70 complex can be again released in the presence of Mg-ATP. In addition, hsp70 binds to K8/18 without having a significant effect on in vitro filament assembly when added during or after assembly. Using an overlay assay, hsp70 binds exclusively to K8 in the presence of ATP. Our results show direct association of the hsp/c70 proteins with K8/18. This interaction may serve, at least in part, to regulate the function of these two abundant protein groups.
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影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ
一种酪氨酸硫酸化人糖蛋白，具有不寻常的细胞分布。
影响因子：
3.6
作者：
Omary,MB;deGrandpre,L;Varki,NM;Kagnoff,MF
通讯作者：
Kagnoff,MF
DOI：
10.1016/s0021-9258(19)36777-8
发表时间：
1992-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Cyr;Xiangyang Lu;M. Douglas
通讯作者：
D. Cyr;Xiangyang Lu;M. Douglas
DOI：
10.1016/s0021-9258(18)54851-1
发表时间：
1991-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Todd A. Wyatt;Thomas M. Lincoln;K. Pryzwansky
通讯作者：
Todd A. Wyatt;Thomas M. Lincoln;K. Pryzwansky
Beta-internexin 是一种微管相关蛋白，与 70-kDa 热休克同源蛋白和网格蛋白脱壳 ATP 酶相同。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Green,LA;Liem,RK
通讯作者：
Liem,RK