Lateral diffusion and signaling of receptor for advanced glycation end-products (RAGE): a receptor involved in chronic inflammation
Lateral diffusion and signaling of receptor for advanced glycation end-products (RAGE): a receptor involved in chronic inflammation
复制标题
晚期糖基化终产物受体 (RAGE) 的横向扩散和信号传导：一种参与慢性炎症的受体
DOI：
10.1007/s00249-017-1227-5
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Smith, Emily A.
中科院分区：
文献类型：
--
作者：
Syed, Aleem;Zhu, Qiaochu;Smith, Emily A.
Membrane diffusion is one of the key mechanisms in the cellular function of receptors. The signaling of receptors for advanced glycation end-products (RAGE) has been extensively studied in the context of several pathological conditions, however, very little is known about RAGE diffusion. To fill this gap, RAGE lateral diffusion is probed in native, cholesterol-depleted, and cytoskeleton-altered cellular conditions. In native GM07373 cellular conditions, RAGE has a 90% mobile fraction and an average diffusion coefficient of 0.3 μm2/s. When depolymerization of the actin cytoskeleton is inhibited with the small molecule jasplakinolide (Jsp), the RAGE mobile fraction and diffusion coefficient decrease by 22 and 37%, respectively. In contrast, depolymerizing the filamentous actin cytoskeleton using the small molecule cytochalasin D (CD) does not alter the RAGE diffusion properties. There is a 70 and 50% decrease in phosphorylation of extracellular signal-regulated kinase (p-ERK) when the actin cytoskeleton is disrupted by CD or Jsp, respectively, in RAGE-expressing GM07373 cells. Disrupting the actin cytoskeleton in GM07373 cells that do not express detectable amounts of RAGE results in no change in p-ERK. Cholesterol depletion results in no statistically significant change in the diffusion properties of RAGE or p-ERK. This work presents a strong link between the actin cytoskeleton and RAGE diffusion and downstream signaling, and serves to further our understanding of the factors influencing RAGE lateral diffusion.
登录
查看更多内容
影响因子：
9.6
作者：
Briot R;Frank JA;Uchida T;Lee JW;Calfee CS;Matthay MA
通讯作者：
Matthay MA
DOI：
10.1016/j.str.2010.05.017
发表时间：
2010-10-13
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Koch M;Chitayat S;Dattilo BM;Schiefner A;Diez J;Chazin WJ;Fritz G
通讯作者：
Fritz G
影响因子：
3.5
作者：
A. Kusumi;Y. M. Shirai;I. Koyama-Honda;K. G. Suzuki;and T. K. Fujiwara
通讯作者：
and T. K. Fujiwara
DOI：
10.1073/pnas.96.16.9095
发表时间：
1999-08
影响因子：
11.1
作者：
M. Shaw;L. Tilney
通讯作者：
M. Shaw;L. Tilney
影响因子：
4.8
作者：
Zong, Hongliang;Madden, Angelina;Stitt, Alan W.
通讯作者：
Stitt, Alan W.