Deposition Bias of Chromatin Proteins Inverts under DNA Replication Stress Conditions.
Deposition Bias of Chromatin Proteins Inverts under DNA Replication Stress Conditions.
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DOI：
10.1021/acschembio.1c00321
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发表时间：
2021-11-19
影响因子：
4
通讯作者：
Dekker FJ
中科院分区：
文献类型：
--
作者：
Zwinderman MRH;Lobo TJ;van der Wouden PE;Spierings DCJ;van Vugt MATM;Lansdorp PM;Guryev V;Dekker FJ
在DNA复制后，通过亲本染色质蛋白的再沉积和新合成的染色质蛋白的沉积，等量的染色质蛋白分布在姐妹染色单体上。平衡新旧染色质蛋白质分配的分子机制在很大程度上仍然未知。在这里，我们研究了新的染色质蛋白相对于亲本DNA模板链和复制起始区的全基因组分布使用双击测序。在控制条件下，新的染色质蛋白质优先被发现的DNA复制的滞后链机械。引人注目的是，复制应激诱导的羟基脲或箭毒治疗和共济失调毛细血管扩张症和Rad3相关蛋白（ATR）或p53失活的抑制反转观察到的染色质蛋白沉积的偏见链复制的领先链聚合酶与先前报道的影响复制蛋白A占用。我们认为，新合成的染色质蛋白质不对称沉积到姐妹染色单体上反映了前导链和滞后链合成的持续合成能力的差异。
Following DNA replication, equal amounts of chromatin proteins are distributed over sister chromatids by re-deposition of parental chromatin proteins and deposition of newly synthesized chromatin proteins. Molecular mechanisms balancing the allocation of new and old chromatin proteins remain largely unknown. Here, we studied the genome-wide distribution of new chromatin proteins relative to parental DNA template strands and replication initiation zones using the double-click-seq. Under control conditions, new chromatin proteins were preferentially found on DNA replicated by the lagging strand machinery. Strikingly, replication stress induced by hydroxyurea or curaxin treatment and inhibition of ataxia telangiectasia and Rad3-related protein (ATR) or p53 inactivation inverted the observed chromatin protein deposition bias to the strand replicated by the leading strand polymerase in line with previously reported effects on replication protein A occupancy. We propose that asymmetric deposition of newly synthesized chromatin proteins onto sister chromatids reflects differences in the processivity of leading and lagging strand synthesis.
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影响因子：
16
作者：
Reverón-Gómez N;González-Aguilera C;Stewart-Morgan KR;Petryk N;Flury V;Graziano S;Johansen JV;Jakobsen JS;Alabert C;Groth A
通讯作者：
Groth A
核苷酸池的耗竭诱导基因表达的G-四链体依赖性扰动。
影响因子：
8.8
作者：
Papadopoulou C;Guilbaud G;Schiavone D;Sale JE
通讯作者：
Sale JE
影响因子：
10.5
作者：
Alabert C;Barth TK;Reverón-Gómez N;Sidoli S;Schmidt A;Jensen ON;Imhof A;Groth A
通讯作者：
Groth A
影响因子：
4.7
作者：
Chapman TR;Kinsella TJ
通讯作者：
Kinsella TJ
影响因子：
8.8
作者：
Klusmann, Ina;Rodewald, Sabrina;Dobbelstein, Matthias
通讯作者：
Dobbelstein, Matthias