In Vivo Adenovirus-Mediated Gene Transfer Via the Pulmonary Artery of Rats
In Vivo Adenovirus-Mediated Gene Transfer Via the Pulmonary Artery of Rats
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体内腺病毒介导的大鼠肺动脉基因转移
DOI：
10.1161/01.res.76.5.701
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发表时间：
1995
影响因子：
20.1
通讯作者：
D. Dichek
中科院分区：
文献类型：
--
作者：
S. Schachtner;J. Rome;R. F. Hoyt;K. Newman;R. Virmani;D. Dichek
摘要肺血管内基因转移是研究肺病理生理学和肺血管疾病基因治疗的一项重要技术。为了评估体内肺动脉基因转移的潜力，我们将腺病毒载体注入Sprague-Dawley大鼠和棉鼠的左肺动脉。通过经皮经导管方法或通过开胸术和肺动脉切开术获得左肺动脉通路。采用开胸手术入路，在载体输注期间和随后的20分钟留置期间，肺动脉流入和肺静脉流出均被闭塞。通过在病毒输注后48至72小时的时间点切除的肺中染色重组基因表达的证据来评估基因转移的成功。通过开胸技术，肺基因转移在15%的存活Sprague-Dawley大鼠和30%的存活SD大鼠中成功。
Abstract Gene transfer into the pulmonary vasculature has the potential to be a powerful technique for both investigation of pulmonary pathophysiology and development of genetic therapies for pulmonary vascular disease. To evaluate the potential for in vivo pulmonary arterial gene transfer, we infused adenoviral vectors into the left pulmonary artery of Sprague-Dawley and cotton rats. Access to the left pulmonary artery was obtained by a percutaneous transcatheter approach or through thoracotomy and pulmonary arteriotomy. With the thoracotomy approach, both pulmonary arterial inflow and pulmonary venous outflow were occluded during vector infusion and throughout a subsequent 20-minute dwell period. The success of gene transfer was assessed by staining for evidence of recombinant gene expression in lungs excised at time points ranging from 48 to 72 hours after virus infusion. With the thoracotomy technique, pulmonary gene transfer was successful in 15% of surviving Sprague-Dawley rats and 30% of surviving ...
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绵羊持续空气栓塞诱导的胰岛素样生长因子 I 和肺动脉高压。
DOI：
10.1165/ajrcmb/6.1.82
发表时间：
1992
影响因子：
6.4
作者：
Perkett,EA;Badesch,DB;Roessler,MK;Stenmark,KR;Meyrick,B
通讯作者：
Meyrick,B
影响因子：
2.6
作者：
RobertsJr,JD;Shaul,PW
通讯作者：
Shaul,PW
影响因子：
--
作者：
ELTON, TS;OPARIL, S;CHEN, YF
通讯作者：
CHEN, YF
影响因子：
5.1
作者：
ROSENBERG, AA;KENNAUGH, J;ABMAN, SH
通讯作者：
ABMAN, SH
DOI：
10.1073/pnas.86.10.3823
发表时间：
1989-05-01
影响因子：
11.1
作者：
GINSBERG, HS;LUNDHOLMBEAUCHAMP, U;PRINCE, GA
通讯作者：
PRINCE, GA