Application of optogenetics and in vivo imaging approaches for elucidating the neurobiology of addiction.
Application of optogenetics and in vivo imaging approaches for elucidating the neurobiology of addiction.
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DOI：
10.1038/s41380-021-01181-3
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发表时间：
2022-01
影响因子：
11
通讯作者：
Liu, Qing-song
中科院分区：
文献类型：
--
作者：
Vickstrom, Casey R.;Snarrenberg, Shana Terai;Friedman, Vladislav;Liu, Qing-song
50多年来，成瘾的神经生物学一直是一个研究热点。在此期间，研究大脑功能方法的技术创新迅速发展，导致越来越复杂的实验方法。为了了解特定的大脑区域、细胞类型和回路如何受到药物滥用和成瘾行为特征的影响，有必要观察和操纵成瘾相关行为范式中的神经活动。为了实现这一目标，近年来在体内成像和神经回路调制方面取得了几项关键技术进步，这些技术已经阐明了成瘾的细胞和回路机制。在这里，我们讨论了其中的一些关键技术，包括光遗传学的电路调制，微型单光子显微镜（miniscope）和纤维光度法的体内成像，以及这些技术的应用如何为成瘾的神经生物学提供新的见解。
The neurobiology of addiction has been an intense topic of investigation for more than 50 years. Over this time, technological innovation in methods for studying brain function rapidly progressed, leading to increasingly sophisticated experimental approaches. To understand how specific brain regions, cell types, and circuits are affected by drugs of abuse and drive behaviors characteristic of addiction, it is necessary both to observe and manipulate neural activity in addiction-related behavioral paradigms. In pursuit of this goal, there have been several key technological advancements in in vivo imaging and neural circuit modulation in recent years, which have shed light on the cellular and circuit mechanisms of addiction. Here we discuss some of these key technologies, including circuit modulation with optogenetics, in vivo imaging with miniaturized single-photon microscopy (miniscope) and fiber photometry, and how the application of these technologies has garnered novel insights into the neurobiology of addiction.
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