High-performance liquid chromatographic column packings with different particle sizes: chromatographic behavior for the quality analysis of HuanglianShangqing pill.
High-performance liquid chromatographic column packings with different particle sizes: chromatographic behavior for the quality analysis of HuanglianShangqing pill.
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不同粒径高效液相色谱柱填料：黄连上清丸质量分析的色谱行为
DOI：
10.1002/jssc.201400981
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Daoquan Tang
中科院分区：
文献类型：
--
作者：
Yan Du;Yinjie Li;Xiaoxiao Zheng;Daoquan Tang
The chromatographic separation of traditional Chinese medicines is still a highly challenging task in analytical science with respect to its hundreds and thousands of chemical compounds, while increase of separation efficiency can greatly improve the separation power of chromatographic column for traditional Chinese medicine. In this study, 13 bioactive components in HuanglianShangqing pill were selected as an index to optimize the separation conditions and evaluate the system suitability of three commercially available columns packed with 1.8, 3.5, and 5.0 μm particles. The chromatographic separations were obtained by the most appropriate Eclipse Plus C18 column (100 × 2.1 mm, 3.5 μm) within 45 min using gradient elution with aqueous-ammonium acetate (10 mmol/L, pH 5.0) and acetonitrile, at a flow rate of 0.3 mL/min and an operating temperature of 30°C. The quality of HuanglianShangqing pill was assessed through combining simultaneous quantification of 13 compounds with fingerprint analysis. For the qualitative analysis, mass spectrometry was used to confirm the 13 compounds. All the validation data conformed to the acceptable requirements. For the fingerprint analysis, 32 peaks were selected as the common peaks at 254 nm to evaluate the similarities among HuanglianShangqing pills obtained from ten manufacturers.
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影响因子：
6.1
作者：
Yang, Dong-Zhi;An, Ti Qiang;Wu, Xiao-Wen
通讯作者：
Wu, Xiao-Wen
影响因子：
3.1
作者：
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通讯作者：
Chen, Shilin
DOI：
10.1016/j.jpba.2013.05.039
发表时间：
2013-10-01
影响因子：
3.4
作者：
Fan, Chunlin;Deng, Jiewei;Ye, Wencai
通讯作者：
Ye, Wencai
影响因子：
3.1
作者：
Tang, Dao-quan;Li, Zheng;Yang, Dong-zhi
通讯作者：
Yang, Dong-zhi
影响因子：
3.1
作者：
N. Wu;Ashley C Bradley;C. Welch;Li Zhang
通讯作者：
N. Wu;Ashley C Bradley;C. Welch;Li Zhang