Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes.
Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes.
复制标题
DOI：
10.1083/jcb.201201024
复制
发表时间：
2012-07-09
期刊：
影响因子：
--
通讯作者：
Weisman LS
中科院分区：
文献类型：
--
作者：
Eves PT;Jin Y;Brunner M;Weisman LS
Myo 2上的一个重叠的结合位点，以确定这些细胞器在出芽过程中的遗传竞争的cargo适配器和cargo适配器。在细胞分裂过程中，细胞器在特定的时间分布到不同的位置。对于酵母空泡，肌球蛋白V马达Myo 2及其空泡特异性货物适配器Vac 17调节空泡沉积的位置和空泡运动的时间。在本文中，我们表明，Mmr 1的功能作为一个特定的货物适配器在细胞周期的早期，Mmr 1结合Myo 2的网站，结合Vac 17。我们证明，Vac 17和Mmr 1竞争结合在这个网站上。出乎意料的是，这种竞争调节子细胞继承的空泡和线粒体的体积。此外，九个已知的Myo 2货物衔接子中的八个在两个位点之一重叠。Vac 17和Mmr 1在一个位点重叠，而Ypt 11和Kar 9结合的残基亚组也结合Ypt 31/Ypt 32，Sec 4和Inp 2。这些观察结果预测，竞争获得Myo 2可能是一个共同的机制，以协调不同货物的继承。
Vacuole- and mitochondria-specific cargo adaptors compete for an overlapping binding site on Myo2 to determine the inheritance of these organelles during budding. During cell division, organelles are distributed to distinct locations at specific times. For the yeast vacuole, the myosin V motor, Myo2, and its vacuole-specific cargo adaptor, Vac17, regulate where the vacuole is deposited and the timing of vacuole movement. In this paper, we show that Mmr1 functions as a mitochondria-specific cargo adaptor early in the cell cycle and that Mmr1 binds Myo2 at the site that binds Vac17. We demonstrate that Vac17 and Mmr1 compete for binding at this site. Unexpectedly, this competition regulates the volume of vacuoles and mitochondria inherited by the daughter cell. Furthermore, eight of the nine known Myo2 cargo adaptors overlap at one of two sites. Vac17 and Mmr1 overlap at one site, whereas Ypt11 and Kar9 bind subsets of residues that also bind Ypt31/Ypt32, Sec4, and Inp2. These observations predict that competition for access to Myo2 may be a common mechanism to coordinate the inheritance of diverse cargoes.
登录
查看更多内容
影响因子：
9.2
作者：
Beach, DL;Thibodeaux, J;Bloom, K
通讯作者：
Bloom, K
影响因子：
9.2
作者：
Arai, Seisuke;Noda, Yoichi;Yoda, Koji
通讯作者：
Yoda, Koji
酵母肌球蛋白-V尾部域中的两个不同区域是不同货物的移动所必需的。
影响因子：
7.8
作者：
Catlett, N L;Duex, J E;Tang, F;Weisman, L S
通讯作者：
Weisman, L S
影响因子：
11.8
作者：
Jin, Yui;Sultana, Azmiri;Gandhi, Pallavi;Franklin, Edward;Hamamoto, Susan;Khan, Amir R.;Munson, Mary;Schekman, Randy;Weisman, Lois S.
通讯作者：
Weisman, Lois S.
DOI：
10.1083/jcb.200904050
发表时间：
2009-08-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fagarasanu A;Mast FD;Knoblach B;Jin Y;Brunner MJ;Logan MR;Glover JN;Eitzen GA;Aitchison JD;Weisman LS;Rachubinski RA
通讯作者：
Rachubinski RA