Down-regulation of four putative arabinoxylan feruloyl transferase genes from family PF02458 reduces ester-linked ferulate content in rice cell walls.
Down-regulation of four putative arabinoxylan feruloyl transferase genes from family PF02458 reduces ester-linked ferulate content in rice cell walls.
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DOI：
10.1007/s00425-009-1077-1
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发表时间：
2010-02
期刊：
影响因子：
4.3
通讯作者：
Dubcovsky J
中科院分区：
文献类型：
--
作者：
Piston F;Uauy C;Fu L;Langston J;Labavitch J;Dubcovsky J
从木质纤维素材料生产乙醇的工业工艺是可行的，但必须提高效率才能使其在经济上可行。木质纤维素转化为乙醇的限制之一是在紧密的细胞壁基质内难以接近纤维素和半纤维素。阿魏酸 (FA) 可以通过二阿魏酸和寡阿魏酸桥交联草细胞壁中的不同阿拉伯木聚糖分子。这种复杂的交联被认为是限制草细胞壁生物降解性的关键因素，因此，减少 FA 是提高酶对纤维素和半纤维素的可及性的一个有吸引力的目标。不幸的是，我们对负责 FA 掺入细胞壁的基因的了解是有限的。基于草类相对于双子叶植物的基因相似性和更高的转录本丰度的生物信息学预测表明，来自 pfam 家族 PF02458 的基因可能充当阿拉伯木聚糖阿魏酰基转移酶。我们在这里表明，茎中细胞壁中的 FA 含量以及水稻基因 Os05g08640、Os06g39470、Os01g09010 和 Os06g39390 的转录水平均高于叶中。此外，同时下调这四个基因转录水平的 RNA 干扰 (RNAi) 构建体与转基因植物叶子细胞壁 FA 相对于对照的显着减少相关（减少 19%，P < 0.0001）。因此，我们在水稻中的实验结果支持了生物信息学预测，即 PF02458 家族成员参与了 FA 掺入草类细胞壁。本文的在线版本 (doi:10.1007/s00425-009-1077-1) 包含补充材料，可供授权用户使用。
Industrial processes to produce ethanol from lignocellulosic materials are available, but improved efficiency is necessary to make them economically viable. One of the limitations for lignocellulosic conversion to ethanol is the inaccessibility of the cellulose and hemicelluloses within the tight cell wall matrix. Ferulates (FA) can cross-link different arabinoxylan molecules in the cell wall of grasses via diferulate and oligoferulate bridges. This complex cross-linking is thought to be a key factor in limiting the biodegradability of grass cell walls and, therefore, the reduction in FA is an attractive target to improve enzyme accessibility to cellulose and hemicelluloses. Unfortunately, our knowledge of the genes responsible for the incorporation of FA to the cell wall is limited. A bioinformatics prediction based on the gene similarities and higher transcript abundance in grasses relative to dicot species suggested that genes from the pfam family PF02458 may act as arabinoxylan feruloyl transferases. We show here that the FA content in the cell walls and the transcript levels of rice genes Os05g08640, Os06g39470, Os01g09010 and Os06g39390, are both higher in the stems than in the leaves. In addition, an RNA interference (RNAi) construct that simultaneously down-regulates transcript levels of these four genes is associated with a significant reduction in FA of the cell walls from the leaves of the transgenic plants relative to the control (19% reduction, P < 0.0001). Therefore, our experimental results in rice support the bioinformatics prediction that members of family PF02458 are involved in the incorporation of FA into the cell wall in grasses. The online version of this article (doi:10.1007/s00425-009-1077-1) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
GRABBER, JH;HATFIELD, RD;AMRHEIN, N
通讯作者：
AMRHEIN, N
影响因子：
6
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作者：
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影响因子：
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作者：
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