Acute physical activity effects on cardiac gene expression.
Acute physical activity effects on cardiac gene expression.
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DOI：
10.1113/expphysiol.2010.054858
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发表时间：
2010-11
影响因子：
2.7
通讯作者：
Hagerman AE
中科院分区：
文献类型：
--
作者：
Simonsen ML;Alessio HM;White P;Newsom DL;Hagerman AE
定期的体力活动可能会导致基因表达的变化，这些变化随着时间的推移而积累，并最终影响表型，如体重，血脂谱和肿瘤的发展。此外，急性活动可能会影响基因表达和表型不同，这取决于个人是否经常不活跃或活跃。将1月龄雄性Sprague-Dawley大鼠（n=72）等分为SED（标准实验室笼，n=24）、PA（大活动箱，n=24）和EX（标准笼内的运动轮，n=24）组。在三个月大时，每组中一半的动物在休息时被处死，另一半在30分钟的体力活动后被处死。从心脏组织中提取RNA，并对27，000个基因进行微阵列分析。使用qPCR验证选择基因结果。在休息时比较所有3月龄组时，没有发生基因表达差异。在所有组中，在急性游泳活动后，相对较小百分比的基因（1.9%）差异表达（p<0.05），但只有37个独特的和可识别的基因达到或超过两倍的表达差异。急性活动后，基因Atf 3、Fos、Apold 1和Pxdn在SED、PA和EX中表达不同，基因表达的幅度明显分离，SED > PA > EX。年轻的身体不活跃和活跃的动物急性活动后的基因表达水平的差异在影响健康相关表型的基因网络中具有不同的调节作用。
Regular bouts of physical activity may cause changes in gene expression that accumulate over time and ultimately affect phenotypes, such as body weight, blood lipid profile, and tumor development. Furthermore, acute activity may affect gene expression and phenotypes differently depending on whether the individual is regularly inactive or active. One-month old male Sprague-Dawley rats (n=72) were equally divided into SED (standard laboratory cage, n=24), PA (large activity box, n=24), and EX (exercise wheel inside standard cage, n=24) groups. At three months of age, half the animals from each group were sacrificed at rest and the other half following 30 minutes of physical activity. RNA was extracted from cardiac tissue, and microarray analysis was performed on 27,000 genes. Select gene results were validated using qPCR. No gene expression differences occurred when comparing all 3-month old groups at rest. A relatively small percentage of genes (1.9%) were differentially expressed (p<0.05) following acute swim activity in all groups but only 37 unique and identifiable genes reached or exceeded two fold differences in expression. The genes Atf3, Fos, Apold1, and Pxdn were expressed differently among SED, PA and EX following acute activity, with a clear separation of the magnitude in gene expression with SED > PA > EX. Differences in gene expression levels in young physically inactive and active animals following acute activity have different regulatory roles in gene networks that affect health-related phenotypes.
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影响因子：
3.7
作者：
Collins A;Hill LE;Chandramohan Y;Whitcomb D;Droste SK;Reul JM
通讯作者：
Reul JM
影响因子：
7.7
作者：
Hamilton, Marc T.;Hamilton, Deborah G.;Zderic, Theodore W.
通讯作者：
Zderic, Theodore W.
DOI：
10.1073/pnas.91.20.9607
发表时间：
1994-09-27
影响因子：
11.1
作者：
PLUMP, AS;SCOTT, CJ;BRESLOW, JL
通讯作者：
BRESLOW, JL
影响因子：
5.6
作者：
Catalano, Stefania;Giordano, Cinzia;Ando, Sebastiano
通讯作者：
Ando, Sebastiano
影响因子：
5.5
作者：
Bey, L;Hamilton, MT
通讯作者：
Hamilton, MT