Isolation, culture and long-term maintenance of primary mesencephalic dopaminergic neurons from embryonic rodent brains.
Isolation, culture and long-term maintenance of primary mesencephalic dopaminergic neurons from embryonic rodent brains.
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来自胚胎啮齿动物大脑的原代中脑多巴胺能神经元的分离、培养和长期维持。
DOI：
10.3791/52475
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kambiz N. Alavian
中科院分区：
文献类型：
--
作者：
M. Weinert;Tharakeswari Selvakumar;T. Tierney;Kambiz N. Alavian
中脑多巴胺能（mesDA）神经元变性是帕金森病的病理标志。研究这些神经元的生理功能和脆弱性和死亡所涉及的生物学过程，对于理解这种常见的神经退行性疾病的根本原因和解开治疗方法是非常重要的。原代培养的mesDA神经元提供了一个工具，调查的分子，生物化学和电生理特性，以了解这些神经元的发展，长期生存和退化过程中的疾病。本文介绍了从E12.5小鼠（或E14.5大鼠）胚胎中分离、培养和维持中脑多巴胺能神经元的详细方法。优化的细胞培养条件，在这个协议的结果在轴突和树突状突起，突触连接和其他神经元形态学特性的存在下，这使得文化适合于研究的生理，细胞生物学和分子特征的神经元群体。
Degeneration of mesencephalic dopaminergic (mesDA) neurons is the pathological hallmark of Parkinson's diseae. Study of the biological processes involved in physiological functions and vulnerability and death of these neurons is imparative to understanding the underlying causes and unraveling the cure for this common neurodegenerative disorder. Primary cultures of mesDA neurons provide a tool for investigation of the molecular, biochemical and electrophysiological properties, in order to understand the development, long-term survival and degeneration of these neurons during the course of disease. Here we present a detailed method for the isolation, culturing and maintenance of midbrain dopaminergic neurons from E12.5 mouse (or E14.5 rat) embryos. Optimized cell culture conditions in this protocol result in presence of axonal and dendritic projections, synaptic connections and other neuronal morphological properties, which make the cultures suitable for study of the physiological, cell biological and molecular characteristics of this neuronal population.
DOI：
10.1073/pnas.89.19.8943
发表时间：
1992-10-01
影响因子：
11.1
作者：
CHOI, HK;WON, L;HELLER, A
通讯作者：
HELLER, A