Weak power frequency magnetic field acting similarly to EGF stimulation, induces acute activations of the EGFR sensitive actin cytoskeleton motility in human amniotic cells.
Weak power frequency magnetic field acting similarly to EGF stimulation, induces acute activations of the EGFR sensitive actin cytoskeleton motility in human amniotic cells.
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DOI：
10.1371/journal.pone.0087626
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xia RH
中科院分区：
文献类型：
--
作者：
Wu X;Cao MP;Shen YY;Chu KP;Tao WB;Song WT;Liu LP;Wang XH;Zheng YF;Chen SD;Zeng QL;Xia RH
在本文中，我们研究了弱工频磁场（MF）对表皮生长因子受体（EGFR）敏感运动机制的运动相关影响，包括F-肌动蛋白细胞骨架、侵入性突起的生长以及人羊膜上皮（FL）细胞中信号分子的水平。在没有细胞外 EGF 刺激的情况下，电场会刺激新突起的大量生长，特别是丝状伪足和片状伪足，即纽蛋白相关粘着斑数量的增加。并且，发现细胞中心的应力纤维含量明显减少，对应于更大的细胞表面积和体外FL细胞肌动蛋白组装效率的降低，这与总体F-肌动蛋白含量和特殊分布的减少有关。这些效应还与 EGFR 下游运动相关信号分子的蛋白质含量或分布模式的变化有关。所有这些效应与表皮生长因子 (EGF) 刺激细胞后的效应相似，并且具有时间依赖性。这些结果表明，工频 MF 暴露会严重影响由 EGFR 细胞骨架信号通路调节的迁移/运动相关的肌动蛋白细胞骨架重组。因此，在暴露于 MF 后，细胞可能会发生变化，准备好转入迁移状态以响应刺激。
In this article, we have examined the motility-related effects of weak power frequency magnetic fields (MFs) on the epidermal growth factor receptor (EGFR)-sensitive motility mechanism, including the F-actin cytoskeleton, growth of invasive protrusions and the levels of signal molecules in human amniotic epithelial (FL) cells. Without extracellular EGF stimulation, the field stimulated a large growth of new protrusions, especially filopodia and lamellipodia, an increased population of vinculin-associated focal adhesions. And, an obvious reduction of stress fiber content in cell centers was found, corresponding to larger cell surface areas and decreased efficiency of actin assembly of FL cells in vitro, which was associated with a decrease in overall F-actin content and special distributions. These effects were also associated with changes in protein content or distribution patterns of the EGFR downstream motility-related signaling molecules. All of these effects are similar to those following epidermal growth factor (EGF) stimulation of the cells and are time dependent. These results suggest that power frequency MF exposure acutely affects the migration/motility-related actin cytoskeleton reorganization that is regulated by the EGFR-cytoskeleton signaling pathway. Therefore, upon the MF exposure, cells are likely altered to be ready to transfer into a state of migration in response to the stimuli.
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DOI：
10.1083/jcb.119.2.349
发表时间：
1992-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
den Hartigh JC;van Bergen en Henegouwen PM;Verkleij AJ;Boonstra J
通讯作者：
Boonstra J
DOI：
10.1002/jez.a.359
发表时间：
2007-03-01
影响因子：
2.8
作者：
Komazaki, Shinji;Takano, Kazuhiro
通讯作者：
Takano, Kazuhiro
影响因子：
1.9
作者：
Jia, Caili;Zhou, Zhenjie;Xia, Ruohong
通讯作者：
Xia, Ruohong
影响因子：
3.9
作者：
Fan, Lifei;Mellor, Harry
通讯作者：
Mellor, Harry
影响因子：
5.5
作者：
Heo, JS;Lee, YJ;Han, HJ
通讯作者：
Han, HJ